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Literature summary extracted from

  • Nakamura, T.; Iwahashi, A.; Eguchi, Y.
    Enzymatic proof for the identity of the S-sulfocysteine synthase and cysteine synthase B of Salmonella typhimurium (1984), J. Bacteriol., 158, 1122-1127.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.47 D-cycloserine 82% loss of activity at 5 mM Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 NH2OH 97% loss of activity at 10 mM Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 S-sulfocysteine 26% loss of activity at 5 mM Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 Semicarbazide 60% loss of activity at 1 mM Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 3.6
-
 H2S
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 7.1
-
 O-acetyl-L-Ser
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 29000
-
 2 * 29000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 55000
-
 cysteine synthase B, gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.47 O-acetyl-L-Ser + sulfide Salmonella enterica subsp. enterica serovar Typhimurium involved in thiosulfate assimilation L-Cys + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
2.5.1.47 Salmonella enterica subsp. enterica serovar Typhimurium
-
 S-sulfocysteine synthase is identical with cysteine synthase B
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 303
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O-acetyl-L-Ser + H2S
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-Cys + acetate
-
 ?
2.5.1.47 O-acetyl-L-Ser + methyl mercaptan
-
 Salmonella enterica subsp. enterica serovar Typhimurium S-methylcysteine + acetate
-
 ?
2.5.1.47 O-acetyl-L-Ser + sulfide involved in thiosulfate assimilation Salmonella enterica subsp. enterica serovar Typhimurium L-Cys + acetate
-
 ?
2.5.1.47 O-acetyl-L-Ser + thiosulfate
-
 Salmonella enterica subsp. enterica serovar Typhimurium S-sulfocysteine + sodium acetate
-
 ?
2.5.1.47 O-acetyl-L-serine
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-cysteine + acetate
-
 ?
2.5.1.47 O-acetyl-L-serine + thiosulfate
-
 Salmonella enterica subsp. enterica serovar Typhimurium S-sulfo-L-cysteine + acetate + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 dimer 2 * 29000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.47 65
-
 5 min, unstable above Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 77
-
 5 min, complete loss of activity Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 7.2
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.47 pyridoxal 5'-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium