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Literature summary extracted from

  • Hensel, G.; Trueper, H.G.
    O-Acetylserine sulfhydrylase and S-sulfocysteine synthase activities of Rhodospirillum tenue (1983), Arch. Microbiol., 134, 227-232.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.47 L-cysteine 28-41% inhibition at 4.5 mM, isoenzyme-dependent Rhodocyclus tenuis
2.5.1.47 S-sulfocysteine 24% inhibition at 4.5 mM, isoenzyme 1 Rhodocyclus tenuis
2.5.1.47 Sulfide
-
 Rhodocyclus tenuis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.6
-
 H2S isoenzyme 1 and 2 Rhodocyclus tenuis
2.5.1.47 2.9
-
 O-acetyl-L-Ser isoenzyme 1 Rhodocyclus tenuis
2.5.1.47 50
-
 O-acetyl-L-Ser isoenzyme 2 Rhodocyclus tenuis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 46000
-
 isoenzyme 2, gel filtration Rhodocyclus tenuis
2.5.1.47 57000
-
 isoenzyme 1, gel filtration Rhodocyclus tenuis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.47 O-acetyl-L-Ser + sulfide Rhodocyclus tenuis repressed during growth with sulfide or thiosulfide as sulfur source L-Cys + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Rhodoblastus acidophilus
-
-
-
2.5.1.47 Rhodocyclus purpureus
-
-
-
2.5.1.47 Rhodocyclus tenuis
-
 two isoenzymes, one with additional S-sulfocysteine synthase activity
-
2.5.1.47 Rhodomicrobium vannielii
-
-
-
2.5.1.47 Thiocystis violacea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47
-
 Rhodocyclus tenuis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 130
-
 isoenzyme 1 Rhodocyclus tenuis
2.5.1.47 143
-
 isoenzyme 2 Rhodocyclus tenuis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O-acetyl-L-Ser + sulfide repressed during growth with sulfide or thiosulfide as sulfur source Rhodocyclus tenuis L-Cys + acetate
-
 ?
2.5.1.47 O-acetyl-L-serine
-
 Rhodocyclus tenuis L-cysteine + acetate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 7.3 8.2 isoenzyme 1 Rhodocyclus tenuis
2.5.1.47 7.7
-
-
 Rhodocyclus tenuis