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Literature summary extracted from

  • Hensel, G.; Trueper, H.G.
    O-acetylserine sulfhydrylase and S-sulfocysteine synthase activities of Chromatium vinosum (1981), Arch. Microbiol., 130, 228-233.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.47 L-cysteine 35% inhibition at 4.5 mM Allochromatium vinosum
2.5.1.47 S-sulfocysteine 52% inhibition at 4.5 mM Allochromatium vinosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.4
-
 H2S pH 7.4, 37°C, enzyme 1 Allochromatium vinosum
2.5.1.47 1
-
 O-acetyl-L-serine pH 7.4, 37°C, isoenzyme 1 Allochromatium vinosum
2.5.1.47 1.6
-
 H2S pH 7.4, 37°C, isoenzyme 2 Allochromatium vinosum
2.5.1.47 15
-
 O-acetyl-L-serine pH 7.4, 37°C, isoenzyme 2 Allochromatium vinosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 50000
-
 isoenzyme 2, gel filtration Allochromatium vinosum
2.5.1.47 56000
-
 isoenzyme 1, gel filtration Allochromatium vinosum

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Allochromatium vinosum
-
 two isoenzymes, one with additional S-sulfocysteine synthase activity
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47
-
 Allochromatium vinosum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 2.43
-
 isoenzyme 1 Allochromatium vinosum
2.5.1.47 245.6
-
 isoenzyme 2 Allochromatium vinosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O-acetyl-L-serine
-
 Allochromatium vinosum L-cysteine + acetate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 7.2
-
 isoenzyme 1 Allochromatium vinosum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.47 7.3 8.2 isoenzyme 2 Allochromatium vinosum