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Literature summary extracted from

  • Kredich, N.M.; Becker, M.A.
    Cysteine biosynthesis: serine transacetylase and O-acetylserine sulfhydrylase (1971), Methods Enzymol., 17 B, 459-470.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.1
-
 H2S less than Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 0.12
-
 acetyl-CoA
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 0.12
-
 acetyl-CoA
-
 Escherichia coli
2.5.1.47 0.7
-
 L-Ser
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 0.7
-
 L-Ser
-
 Escherichia coli
2.5.1.47 5
-
 O-acetyl-L-Ser free enzyme Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 20
-
 O-acetyl-L-Ser complex-bound enzyme Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 68000
-
 free enzyme, sedimentation equilibrium Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 309000
-
 cysteine synthetase complex, sedimentation equilibrium Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.47 O-acetyl-L-Ser + sulfide Salmonella enterica subsp. enterica serovar Typhimurium final step in Cys synthesis L-Cys + acetate
-
 ?
2.5.1.47 O-acetyl-L-Ser + sulfide Escherichia coli final step in Cys synthesis L-Cys + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Escherichia coli
-
-
-
2.5.1.47 Salmonella enterica subsp. enterica serovar Typhimurium
-
 one enzyme free, one associated with serine transacetylase in cysteine synthase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 292
-
 complex-bound enzyme Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 1100
-
 free enzyme Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
2.5.1.47 -20 C, Tris-HCl buffer, pH 7.6, bovine serum albumine, 8 months, 10% loss of activity Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 20 C, room temperature, Tris-HCl buffer, pH 7.6, bovine serum albumine, several days, no loss of activity Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 L-Cys + acetate
-
 Salmonella enterica subsp. enterica serovar Typhimurium O-acetyl-L-Ser + H2S
-
 r
2.5.1.47 L-Cys + acetate
-
 Escherichia coli O-acetyl-L-Ser + H2S
-
 r
2.5.1.47 O-acetyl-L-Ser + H2S
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-Cys + acetate
-
 r
2.5.1.47 O-acetyl-L-Ser + H2S
-
 Escherichia coli L-Cys + acetate
-
 r
2.5.1.47 O-acetyl-L-Ser + methyl mercaptan
-
 Salmonella enterica subsp. enterica serovar Typhimurium S-methylcysteine + acetate product identification uncertain ir
2.5.1.47 O-acetyl-L-Ser + sulfide final step in Cys synthesis Salmonella enterica subsp. enterica serovar Typhimurium L-Cys + acetate
-
 ?
2.5.1.47 O-acetyl-L-Ser + sulfide final step in Cys synthesis Escherichia coli L-Cys + acetate
-
 ?
2.5.1.47 O-acetyl-L-serine
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-cysteine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 dimer
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.47 62
-
 10 min, 5% loss of activity Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 67
-
 10 min, 50% loss of activity Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 70
-
 10 min, 70% loss of activity Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.47 733
-
 O-acetyl-L-Ser
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.47 733
-
 O-acetyl-L-Ser
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.47 pyridoxal 5'-phosphate 1 per subunit of free enzyme, 4 per of cysteine synthase complex Salmonella enterica subsp. enterica serovar Typhimurium