Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Legaigneur, P.; Breton, C.; El Battari, A.; Guillemot, J.C.; Auge, C.; Malissard, M.; Berger, E.G.; Ronin, C.
    Exploring the acceptor substrate recognition of the human beta-galactoside alpha 2,6-sialyltransferase (2001), J. Biol. Chem., 276, 21608-21617.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.1 DNA sequence determination, expression of various truncated forms lacking the transmembrane fragment as FLAG-tagged proteins, the chimeric mutant, and the membrane-bound enzyme form in CHO-K1 cells Homo sapiens
2.4.3.1 expression in Pichia pastoris, entry in the secretory pathway, i.e. excretion of the soluble enzyme to the medium, by usage of the N-terminal signal sequence of Saccharomyces cerevisiae alpha-factor, no hyperglycosylation Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.3.1 additional information construction of a chimeric enzyme with the N-terminal membrane portion of the rat core 2 beta1,6-GlcNAc-transferase, amino acid residues 1-52, and the residues 1-70 of the human alpha2,6-sialyltransferase, also FLAG-tagged, functional expression in CHO-K1 cells, broader acceptor substrate specificity than the wild-type Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.1 additional information
-
additional information several recombinant truncated mutants Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.3.1 additional information deletion of the transmembrane fragment results in loss of acceptor preference Homo sapiens
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + asialo-fetuin
-
Homo sapiens CMP + fetuin
-
?
2.4.3.1 CMP-N-acetylneuraminate + asialo-orosomucoid i.e. asialo-alpha1 acid glycoprotein Homo sapiens CMP + orosomucoid
-
?
2.4.3.1 CMP-N-acetylneuraminate + asialo-transferrin
-
Homo sapiens CMP + transferrin
-
?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R acceptor substrate specificity Homo sapiens CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Homo sapiens CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
?
2.4.3.1 additional information the catalytic domain can proceed with sialic acid transfer with increased efficiency until 80 amino acid are deleted Homo sapiens ?
-
?