EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.3.1 | DNA sequence determination, expression of various truncated forms lacking the transmembrane fragment as FLAG-tagged proteins, the chimeric mutant, and the membrane-bound enzyme form in CHO-K1 cells | Homo sapiens |
2.4.3.1 | expression in Pichia pastoris, entry in the secretory pathway, i.e. excretion of the soluble enzyme to the medium, by usage of the N-terminal signal sequence of Saccharomyces cerevisiae alpha-factor, no hyperglycosylation | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.3.1 | additional information | construction of a chimeric enzyme with the N-terminal membrane portion of the rat core 2 beta1,6-GlcNAc-transferase, amino acid residues 1-52, and the residues 1-70 of the human alpha2,6-sialyltransferase, also FLAG-tagged, functional expression in CHO-K1 cells, broader acceptor substrate specificity than the wild-type | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.3.1 | additional information | - |
additional information | several recombinant truncated mutants | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.3.1 | additional information | deletion of the transmembrane fragment results in loss of acceptor preference | Homo sapiens | - |
- |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.3.1 | Homo sapiens | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.3.1 | CMP-N-acetylneuraminate + asialo-fetuin | - |
Homo sapiens | CMP + fetuin | - |
? | |
2.4.3.1 | CMP-N-acetylneuraminate + asialo-orosomucoid | i.e. asialo-alpha1 acid glycoprotein | Homo sapiens | CMP + orosomucoid | - |
? | |
2.4.3.1 | CMP-N-acetylneuraminate + asialo-transferrin | - |
Homo sapiens | CMP + transferrin | - |
? | |
2.4.3.1 | CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | acceptor substrate specificity | Homo sapiens | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
2.4.3.1 | CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | sialic acid is linked to C-6 of the galactose residue | Homo sapiens | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
2.4.3.1 | additional information | the catalytic domain can proceed with sialic acid transfer with increased efficiency until 80 amino acid are deleted | Homo sapiens | ? | - |
? |