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Literature summary extracted from

  • Malissard, M.; Zeng, S.; Berger, E.G.
    Expression of functional soluble forms of human beta-1,4-galactosyltransferase I, alpha-2,6-sialyltransferase, and alpha-1, 3-fucosyltransferase VI in the methylotrophic yeast Pichia pastoris (2000), Biochem. Biophys. Res. Commun., 267, 169-173.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.3.1 biotechnology recombinant enzyme may be used for in vitro synthesis of oligosaccharides Homo sapiens
2.4.3.1 synthesis recombinant enzyme may be used for in vitro synthesis of oligosaccharides Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.1 expression in Pichia pastoris, entry in the secretory pathway, i.e. excretion of the soluble enzyme to the medium, by usage of the N-terminal signal sequence of Saccharomyces cerevisiae alpha-factor, no hyperglycosylation Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.1 0.15
-
 CMP-N-acetylneuraminate recombinant enzyme Homo sapiens
2.4.3.1 2.3
-
 N-acetyllactosamine recombinant enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.3.1 glycoprotein 2times N-glycosylated Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine i.e. N-acetyllactosamine Homo sapiens CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Homo sapiens CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.3.1 37
-
 assay at Homo sapiens