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Literature summary extracted from

  • Paulson, J.C.; Beranek, W.E.; Hill, R.L.
    Purification of a sialyltransferase from bovine colostrum by affinity chromatography on CDP-agarose (1977), J. Biol. Chem., 252, 2356-2362.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.3.1 glycerol, 50%, stabilizes during storage Bos taurus
2.4.3.1 high protein concentrations stabilize Bos taurus
2.4.3.1 use of plastic containers instead of glass stabilizes Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.3.1 cytosol
-
 Bos taurus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.3.1 41500
-
 1 * 41500, low molecular weight form, SDS-PAGE under non-reducing conditions Bos taurus
2.4.3.1 42900
-
 low molecular weight form, sedimentation equilibrium centrifugation, two enzyme forms differing in molecular weight when submitted to gel filtration, the smaller one is probably a degradation product of the larger one Bos taurus
2.4.3.1 44000
-
 1 * 44000, low molecular weight form, SDS-PAGE under reducing conditions Bos taurus
2.4.3.1 45000
-
 low molecular weight form, gel filtration, two enzyme forms differing in molecular weight when submitted to gel filtration, the smaller one is probably a degradation product of the larger one Bos taurus
2.4.3.1 53500
-
 1 * 53500, high molecular weight form, SDS-PAGE under non-reducing conditions Bos taurus
2.4.3.1 56000
-
 1 * 56000, high molecular weight form, SDS-PAGE under reducing conditions Bos taurus
2.4.3.1 57900
-
 high molecular weight form, sedimentation equilibrium centrifugation, two enzyme forms differing in molecular weight when submitted to gel filtration, the smaller one is probably a degradation product of the larger one Bos taurus
2.4.3.1 80000
-
 high molecular weight form, gel filtration, two enzyme forms differing in molecular weight when submitted to gel filtration, the smaller one is probably a degradation product of the larger one Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.1 CDP-hexanolamine-agarose affinity chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.1 colostrum
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.3.1 26 28 purified enzyme Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.4.3.1 -20°C, in 50% glycerol, 12 mM cacodylate, pH 5.3, 2 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + asialo-orosomucoid
-
 Bos taurus CMP + orosomucoid
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.3.1 monomer 1 * 44000, low molecular weight form, SDS-PAGE under reducing conditions Bos taurus
2.4.3.1 monomer 1 * 53500, high molecular weight form, SDS-PAGE under non-reducing conditions Bos taurus
2.4.3.1 monomer 1 * 41500, low molecular weight form, SDS-PAGE under non-reducing conditions Bos taurus
2.4.3.1 monomer 1 * 56000, high molecular weight form, SDS-PAGE under reducing conditions Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.3.1 37
-
 assay at Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.3.1 5.2 5.5 most stable Bos taurus