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Literature summary extracted from

  • Bartholomew, B.A.; Jourdian, G.W.; Roseman, S.
    The sialic acids. XV. Transfer of sialic acid to glycoproteins by a sialyltransferase from colostrum (1973), J. Biol. Chem., 248, 5751-5762.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.3.1 additional information not affected by versenate Capra hircus
2.4.3.1 additional information
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.1 additional information
-
 additional information
-
 Homo sapiens
2.4.3.1 additional information
-
 additional information
-
 Bos taurus
2.4.3.1 additional information
-
 additional information
-
 Capra hircus
2.4.3.1 0.08
-
 CMP-N-acetylneuraminate with lactose Capra hircus
2.4.3.1 0.3
-
 CMP-N-acetylneuraminate with N-acetyllactosamine Capra hircus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.3.1 cytosol
-
 Homo sapiens 5829
-
2.4.3.1 cytosol
-
 Bos taurus 5829
-
2.4.3.1 cytosol
-
 Capra hircus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.3.1 MgCl2 no stimulation Homo sapiens
2.4.3.1 MgCl2 no stimulation Bos taurus
2.4.3.1 MgCl2 no stimulation Capra hircus
2.4.3.1 additional information no metal ion requirement Homo sapiens
2.4.3.1 additional information no metal ion requirement Bos taurus
2.4.3.1 additional information no metal ion requirement Capra hircus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R Homo sapiens final step in synthesis of serum-type glycoproteins CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R Bos taurus final step in synthesis of serum-type glycoproteins CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R Capra hircus final step in synthesis of serum-type glycoproteins CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Bos taurus
-
-
-
2.4.3.1 Capra hircus
-
-
-
2.4.3.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.1 partial Bos taurus
2.4.3.1 partial Capra hircus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.1 colostrum
-
 Homo sapiens
-
2.4.3.1 colostrum
-
 Bos taurus
-
2.4.3.1 colostrum
-
 Capra hircus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.3.1 0.0009
-
 partially purified enzyme Bos taurus
2.4.3.1 0.0035
-
 partially purified enzyme Capra hircus

Storage Stability

EC Number Storage Stability Organism
2.4.3.1 0-4°C, partially purified enzyme preparation, 2-4 weeks Bos taurus
2.4.3.1 0-4°C, partially purified enzyme preparation, 2-4 weeks Capra hircus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + asialo-ceruloplasmin
-
 Capra hircus CMP + ceruloplasmin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-fetuin
-
 Homo sapiens CMP + fetuin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-fetuin
-
 Bos taurus CMP + fetuin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-fetuin
-
 Capra hircus CMP + fetuin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-orosomucoid best substrate Bos taurus CMP + orosomucoid
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-orosomucoid best substrate Capra hircus CMP + orosomucoid
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-orosomucoid i.e. asialo-alpha1 acid glycoprotein Homo sapiens CMP + orosomucoid
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-orosomucoid i.e. asialo-alpha1 acid glycoprotein Bos taurus CMP + orosomucoid
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-orosomucoid i.e. asialo-alpha1 acid glycoprotein Capra hircus CMP + orosomucoid
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-proteose peptone
-
 Capra hircus CMP + proteose peptone
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-prothrombin
-
 Capra hircus CMP + prothrombin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-thyroglobulin low activity Capra hircus CMP + thyroglobulin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-transferrin
-
 Homo sapiens CMP + transferrin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-transferrin
-
 Bos taurus CMP + transferrin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-transferrin
-
 Capra hircus CMP + transferrin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine i.e. N-acetyllactosamine Homo sapiens CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine i.e. N-acetyllactosamine Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine i.e. N-acetyllactosamine Capra hircus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine most active disaccharide substrate Homo sapiens CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine most active disaccharide substrate Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine most active disaccharide substrate Capra hircus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R acceptor substrate specificity Homo sapiens CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R acceptor substrate specificity Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R acceptor substrate specificity Capra hircus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Homo sapiens CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Capra hircus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R high molecular weight substrates are more efficient acceptors than low molecular weight substrates Homo sapiens CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R high molecular weight substrates are more efficient acceptors than low molecular weight substrates Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R high molecular weight substrates are more efficient acceptors than low molecular weight substrates Capra hircus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R final step in synthesis of serum-type glycoproteins Homo sapiens CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R final step in synthesis of serum-type glycoproteins Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R final step in synthesis of serum-type glycoproteins Capra hircus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + fetuin no activity Capra hircus ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + lactose
-
 Homo sapiens CMP + 6'-sialyllactose
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + lactose
-
 Bos taurus CMP + 6'-sialyllactose
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + lactose
-
 Capra hircus CMP + 6'-sialyllactose
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + submaxillary asialo-mucin
-
 Capra hircus CMP + submaxillary mucin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + submaxillary asialo-mucin poor substrates, no activity with ovine acceptor Bos taurus CMP + submaxillary mucin
-
 ?
2.4.3.1 additional information
-
 Homo sapiens ?
-
 ?
2.4.3.1 additional information no activity with ovine submaxillary asialo-mucin Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.3.1 37
-
 assay at Homo sapiens
2.4.3.1 37
-
 assay at Bos taurus
2.4.3.1 37
-
 assay at Capra hircus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.3.1 6.4 7.2 broad, optimal buffer concentration: 0.05-0.15 M, decreasing activity above 0.15 M Capra hircus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.3.1 5.4 8 about half-maximal activity at pH 5.4 and 8, goat Capra hircus