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Literature summary extracted from

  • Weinstein, J.; de Souza-e-Silva, U.; Paulson, J.C.
    Purification of a Gal beta 1-4GlcNAc alpha 2-6 sialyltransferase and a Gal beta 1-3(4)GlcNAc alpha 2-3 sialyltransferase to homogeneity from rat liver (1982), J. Biol. Chem., 257, 13835-13844.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.3.1 Triton CF-54/Tween 80 activation Rattus norvegicus

General Stability

EC Number General Stability Organism
2.4.3.1 high NaCl concentrations stabilize during storage Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.3.1 Golgi apparatus
-
 Rattus norvegicus 5794
-
2.4.3.1 membrane
-
 Rattus norvegicus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.3.1 40500
-
 1 * 40500, SDS-PAGE under reducing conditions Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.1 CDP-hexanolamine-agarose affinity chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.3.1 8.2
-
 purified enzyme Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.4.3.1 -20°C, in 50% glycerol, 35 mM cacodylate, pH 6, 0.45 M NaCl, 0.08% Triton CF-54, at least 1 year Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + asialo-orosomucoid i.e. asialo-alpha1 acid glycoprotein Rattus norvegicus CMP + orosomucoid
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R transfers sialic acid to terminal positions on N-linked glycans Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + lactose
-
 Rattus norvegicus CMP + 6'-sialyllactose
-
 ?
2.4.3.1 additional information no activity with submaxillary asialo-mucin Rattus norvegicus ?
-
 ?
2.4.3.1 additional information no activity with antifreeze glycoprotein Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.3.1 monomer 1 * 40500, SDS-PAGE under reducing conditions Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.3.1 37
-
 assay at Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.3.1 5.3
-
 and below, storage stability decreases with t1/2: about 3 months Rattus norvegicus
2.4.3.1 6
-
 full stability for at least 1 year Rattus norvegicus