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Literature summary extracted from

  • Spiro, M.J.; Spiro, R.G.
    Glycoprotein biosynthesis: studies on thyroglobulin. Thyroid sialyltransferase (1968), J. Biol. Chem., 243, 6520-6528.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.3.1 EDTA no activation Bos taurus

General Stability

EC Number General Stability Organism
2.4.3.1 stable to lyophilization Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.3.1 EDTA no inhibition Bos taurus
2.4.3.1 PCMB weak Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.1 0.27
-
 CMP-N-acetylneuraminate
-
 Bos taurus
2.4.3.1 0.59
-
 asialo-thyroglobulin glycopeptides
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.3.1 membrane
-
 Bos taurus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.3.1 MgCl2 no stimulation Bos taurus
2.4.3.1 additional information no metal ion requirement Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R Bos taurus R: glycoprotein or glycopeptide CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R Bos taurus one of a group of glycosyltransferases which act to assemble the carbohydrate units of thyroglobulin CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Bos taurus
-
 calf
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.1 partial Bos taurus
2.4.3.1 solubilized by ultrasonic treatment Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.1 thyroid gland
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.3.1 additional information
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.4.3.1 frozen, crude, solubilized enzyme preparation, several months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + asialo-fetuin
-
 Bos taurus CMP + fetuin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-thyroglobulin glycopeptide
-
 Bos taurus CMP + thyroglobulin glycopeptide
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R acceptor substrate specificity Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R R: glycoprotein or glycopeptide Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R transfers sialic acid to the terminal galactose residue of asialo-gyloproteins and peptides Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R high molecular weight substrates are more efficient acceptors than low molecular weight substrates Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R one of a group of glycosyltransferases which act to assemble the carbohydrate units of thyroglobulin Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + fetuin glycopeptides Bos taurus ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + lactose no activity Bos taurus CMP + 6'-sialyllactose
-
 ?
2.4.3.1 additional information no activity with galactosylhydroxylysine and its derivatives, or galactose-containing oligosaccharides linked to Ser/Thr-glycopeptide of earthworm cuticle collagen Bos taurus ?
-
 ?
2.4.3.1 additional information no transfer to terminal N-acetyl-D-galactosamine Bos taurus ?
-
 ?
2.4.3.1 additional information no activity with submaxillary asialo-mucin Bos taurus ?
-
 ?
2.4.3.1 additional information galactose-free glycopeptides are poor substrates Bos taurus ?
-
 ?
2.4.3.1 additional information no activity with galactose, lactose and N-acetyllactose Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.3.1 37
-
 assay at Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.3.1 100
-
 5 min, complete inactivation Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.3.1 6
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.3.1 5 9.2 about half-maximal activity at pH 5 and 9.2 Bos taurus