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Literature summary extracted from

  • Hickman, J.; Ashwell, G.; Morell, A.G.; Van den Hamer, C.J.A.; Scheinberg, I.H.
    Physical and chemical studies on ceruloplasmin. 8. Preparation of N-acetylneuraminic acid-1-14C-labeled ceruloplasmin (1970), J. Biol. Chem., 245, 759-766.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.3.1 deoxycholate 9fold stimulation at 0.25%, inhibition at 0.5% Rattus norvegicus
2.4.3.1 EDTA activation Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.3.1 deoxycholate 9fold stimulation at 0.25%, inactivation at 0.5% Rattus norvegicus
2.4.3.1 long chain fatty acids oleic acid, stearic acid, less efficient: palmitic acid, lauric acid, capric acid, caprylic acid or caproic acid, no inhibition by linoleic acid or linolenic acid Rattus norvegicus
2.4.3.1 additional information no inhibition by linoleic acid or linolenic acid Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.1 0.047
-
 CMP-N-acetylneuraminate
-
 Rattus norvegicus
2.4.3.1 0.078
-
 asialo-ceruloplasmin expressed as concentration of acceptor sites Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.3.1 membrane
-
 Rattus norvegicus 16020
-
2.4.3.1 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.3.1 MgCl2 slight stimulation at 5 mM Rattus norvegicus
2.4.3.1 additional information no metal ion requirement Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Rattus norvegicus
-
 Sprague-Dawley
-
2.4.3.1 Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.1 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.3.1 additional information
-
-
 Rattus norvegicus
2.4.3.1 0.00083
-
 partially purified enzyme Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.4.3.1 3°C, microsomal fraction, 2 weeks Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + asialo-apoceruloplasmin best substrate Rattus norvegicus CMP + apoceruloplasmin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-ceruloplasmin
-
 Rattus norvegicus CMP + ceruloplasmin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-chorionic gonadotropin acceptor protein from human source Rattus norvegicus CMP + chorionic gonadotropin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-fetuin
-
 Rattus norvegicus CMP + fetuin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-fetuin
-
 Rattus norvegicus Sprague-Dawley CMP + fetuin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-fibrinogen
-
 Rattus norvegicus CMP + fibrinogen
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-orosomucoid i.e. asialo-alpha1 acid glycoprotein Rattus norvegicus CMP + orosomucoid
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-orosomucoid i.e. asialo-alpha1 acid glycoprotein Rattus norvegicus Sprague-Dawley CMP + orosomucoid
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-thyroglobulin
-
 Rattus norvegicus CMP + thyroglobulin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-transferrin poor substrate Rattus norvegicus CMP + transferrin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R R: glycoprotein or glycopeptide Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R the non-reducing terminal galactosyl residue is essential for activity Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R R: glycoprotein or glycopeptide Rattus norvegicus Sprague-Dawley CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 additional information thyroglobin is no substrate Rattus norvegicus ?
-
 ?
2.4.3.1 additional information no activity with submaxillary asialo-mucin Rattus norvegicus ?
-
 ?
2.4.3.1 additional information thyroglobin is no substrate Rattus norvegicus Sprague-Dawley ?
-
 ?
2.4.3.1 additional information no activity with submaxillary asialo-mucin Rattus norvegicus Sprague-Dawley ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.3.1 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.3.1 6 6.5 2-(N-morpholino)ethane sulfonic acid buffer Rattus norvegicus
2.4.3.1 7.5
-
 Tris-HCl buffer Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.3.1 5 9.7 about 65% of maximal activity at pH 5 and about half-maximal activity at pH 9.7 Rattus norvegicus