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Literature summary extracted from

  • Izumi, Y.; Sato, K.; Tani, Y.; Ogata, K.
    Biosynthesis of biotin by microorganisms. XV. 7,8-Diaminopelargonic acid aminotransferase, an enzyme involved in biotin biosynthesis by microorganisms (1975), Agric. Biol. Chem., 39, 175-181.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.62 7-Keto-8-aminopelargonic acid substrate inhibition above 0.1 mM Corynebacterium glutamicum
2.6.1.62 7-Keto-8-aminopelargonic acid
-
 Escherichia coli
2.6.1.62 As5+
-
 Corynebacterium glutamicum
2.6.1.62 Ca2+
-
 Corynebacterium glutamicum
2.6.1.62 Co2+ 94% inhibition at 1 mM Corynebacterium glutamicum
2.6.1.62 D-cycloserine
-
 Corynebacterium glutamicum
2.6.1.62 DL-Penicillamine
-
 Corynebacterium glutamicum
2.6.1.62 HgCl2 100% inhibition at 1 mM Corynebacterium glutamicum
2.6.1.62 hydroxylamine 100% inhibition at 1 mM Corynebacterium glutamicum
2.6.1.62 iodoacetate
-
 Corynebacterium glutamicum
2.6.1.62 isoniazid 94% inhibition at 1 mM Corynebacterium glutamicum
2.6.1.62 KCN
-
 Corynebacterium glutamicum
2.6.1.62 p-chloromercuribenzoate 100% inhibition at 1 mM Corynebacterium glutamicum
2.6.1.62 Pb2+
-
 Corynebacterium glutamicum
2.6.1.62 phenylhydrazine 100% inhibition at 1 mM Corynebacterium glutamicum
2.6.1.62 Semicarbazide 100% inhibition at 1 mM Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.62 0.00083
-
 pyridoxal 5'-phosphate pH 8, 37°C Corynebacterium glutamicum
2.6.1.62 0.0012
-
 pyridoxamine 5'-phosphate pH 8, 37°C Corynebacterium glutamicum
2.6.1.62 0.069
-
 8-Amino-7-oxononanoate pH 8, 37°C Corynebacterium glutamicum
2.6.1.62 0.55
-
 S-adenosyl-L-methionine pH 8, 37°C Corynebacterium glutamicum
2.6.1.62 1.1
-
 7-amino-8-oxononanoate pH 8, 37°C Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.62 24000
-
 sedimentation equilibrium method Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate Salmonella enterica subsp. enterica serovar Typhimurium biotin synthesis S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate Escherichia coli biotin synthesis S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate Kocuria rosea biotin synthesis S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate Bacillus roseus biotin synthesis S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate Corynebacterium glutamicum biotin synthesis S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate Escherichia coli AKV 0015 biotin synthesis S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate Corynebacterium glutamicum NRRL 2311 biotin synthesis S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate Bacillus roseus IAM 1257 biotin synthesis S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.62 Bacillus roseus
-
 IAM 1257
-
2.6.1.62 Bacillus roseus IAM 1257
-
 IAM 1257
-
2.6.1.62 Corynebacterium glutamicum
-
 NRRL 2311
-
2.6.1.62 Corynebacterium glutamicum NRRL 2311
-
 NRRL 2311
-
2.6.1.62 Escherichia coli
-
 AKV 0015
-
2.6.1.62 Escherichia coli AKV 0015
-
 AKV 0015
-
2.6.1.62 Kocuria rosea
-
 IFO 3764
-
2.6.1.62 Salmonella enterica subsp. enterica serovar Typhimurium
-
 AJ 2635, AJ 2636
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.62 homogeneity Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.62 S-adenosyl-L-methionine + 7-amino-8-oxononanoate 1% of the activity of 8-amino-7-oxononanoate Escherichia coli S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 7-amino-8-oxononanoate 1% of the activity of 8-amino-7-oxononanoate Corynebacterium glutamicum S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 7-amino-8-oxononanoate 1% of the activity of 8-amino-7-oxononanoate Escherichia coli AKV 0015 S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 7-amino-8-oxononanoate 1% of the activity of 8-amino-7-oxononanoate Corynebacterium glutamicum NRRL 2311 S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate synonym: 7-keto-8-aminopelargonic acid, KAPA Salmonella enterica subsp. enterica serovar Typhimurium S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate synonym: 7-keto-8-aminopelargonic acid, KAPA Escherichia coli S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate synonym: 7-keto-8-aminopelargonic acid, KAPA Kocuria rosea S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate synonym: 7-keto-8-aminopelargonic acid, KAPA Bacillus roseus S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate synonym: 7-keto-8-aminopelargonic acid, KAPA Corynebacterium glutamicum S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate biotin synthesis Salmonella enterica subsp. enterica serovar Typhimurium S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate biotin synthesis Escherichia coli S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate biotin synthesis Kocuria rosea S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate biotin synthesis Bacillus roseus S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate biotin synthesis Corynebacterium glutamicum S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate synonym: 7-keto-8-aminopelargonic acid, KAPA Escherichia coli AKV 0015 S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate biotin synthesis Escherichia coli AKV 0015 S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate synonym: 7-keto-8-aminopelargonic acid, KAPA Corynebacterium glutamicum NRRL 2311 S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate biotin synthesis Corynebacterium glutamicum NRRL 2311 S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate synonym: 7-keto-8-aminopelargonic acid, KAPA Bacillus roseus IAM 1257 S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate biotin synthesis Bacillus roseus IAM 1257 S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.62 37
-
-
 Escherichia coli
2.6.1.62 37
-
-
 Corynebacterium glutamicum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.62 25 50 25°C: about 65% of activity maximum, 50°C: about 60% of activity maximum Corynebacterium glutamicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.62 7 60 10 min, stable Corynebacterium glutamicum
2.6.1.62 70
-
 10 min, complete loss of activity Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.62 8.5
-
-
 Escherichia coli
2.6.1.62 8.5
-
-
 Corynebacterium glutamicum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.62 7 11 30 min, stable Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.62 pyridoxal 5'-phosphate cofactor Salmonella enterica subsp. enterica serovar Typhimurium
2.6.1.62 pyridoxal 5'-phosphate cofactor Escherichia coli
2.6.1.62 pyridoxal 5'-phosphate cofactor Kocuria rosea
2.6.1.62 pyridoxal 5'-phosphate cofactor Bacillus roseus
2.6.1.62 pyridoxal 5'-phosphate cofactor Corynebacterium glutamicum
2.6.1.62 pyridoxal 5'-phosphate Km: 0.00083 mM Corynebacterium glutamicum
2.6.1.62 pyridoxamine 5'-phosphate Km: 0.0012 mM Corynebacterium glutamicum
2.6.1.62 pyridoxamine 5'-phosphate active as cofactor Salmonella enterica subsp. enterica serovar Typhimurium
2.6.1.62 pyridoxamine 5'-phosphate active as cofactor Escherichia coli
2.6.1.62 pyridoxamine 5'-phosphate active as cofactor Kocuria rosea
2.6.1.62 pyridoxamine 5'-phosphate active as cofactor Bacillus roseus
2.6.1.62 pyridoxamine 5'-phosphate active as cofactor Corynebacterium glutamicum