Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Alpers, J.B.
    Phosphoribokinase from Pseudomonas saccharophila (1975), Methods Enzymol., 42, 120-123.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.18 ATP above 2 mM Pelomonas saccharophila
2.7.1.18 EDTA reversed by Mg2+ Pelomonas saccharophila
2.7.1.18 ribose 5-phosphate above 2 mM Pelomonas saccharophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.18 1
-
 ATP cosubstrate: ribose 5-phosphate, pH 9.1, 37°C Pelomonas saccharophila
2.7.1.18 1
-
 ribose 5-phosphate cosubstrate: ATP, pH 9.1, 37°C Pelomonas saccharophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.18 ATP + D-ribose 5-phosphate Pelomonas saccharophila
-
 D-ribose 1,5-bisphosphate + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.18 Pelomonas saccharophila
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.18
-
 Pelomonas saccharophila

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.18 9.86
-
-
 Pelomonas saccharophila

Storage Stability

EC Number Storage Stability Organism
2.7.1.18 -56°C, 33 mM sodium phosphate buffer, pH 7.0, 6 months, 25% loss of activity Pelomonas saccharophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.18 ATP + D-ribose 5-phosphate
-
 Pelomonas saccharophila D-ribose 1,5-bisphosphate + ADP
-
 ?
2.7.1.18 ATP + D-ribose 5-phosphate adenosine tetraphosphate can substitute for ATP with 25% residual activity Pelomonas saccharophila D-ribose 1,5-bisphosphate + ADP
-
 ?
2.7.1.18 additional information no substrates: ribose 1-phosphate, glucose 6-phosphate, glucose 1-phosphate, ITP, UTP, ADP, 3',5'-cAMP Pelomonas saccharophila ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.18 9.1
-
-
 Pelomonas saccharophila