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Literature summary extracted from

  • Der Garabedian, P.A.; Lotti, A.M.; Vermeersch, J.J.
    4-Aminobutyrate:2-oxoglutarate aminotransferase from Candida. Purification and properties (1986), Eur. J. Biochem., 156, 589-596.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.19 2-oxoglutarate protects against heat inactivation, better than pyridoxal phosphate Candida guilliermondii var. membranaefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.19 2-aminobutanoate
-
 Candida guilliermondii var. membranaefaciens
2.6.1.19 2-oxoglutarate
-
 Candida guilliermondii var. membranaefaciens
2.6.1.19 alpha-alanine
-
 Candida guilliermondii var. membranaefaciens
2.6.1.19 beta-Alanine
-
 Candida guilliermondii var. membranaefaciens
2.6.1.19 Butyric acid
-
 Candida guilliermondii var. membranaefaciens
2.6.1.19 propionic acid
-
 Candida guilliermondii var. membranaefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.19 1.5
-
 2-oxoglutarate
-
 Candida guilliermondii var. membranaefaciens
2.6.1.19 2.2 2.3 4-aminobutanoate
-
 Candida guilliermondii var. membranaefaciens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.19 cytoplasm
-
 Candida guilliermondii var. membranaefaciens 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.19 107000
-
 gel filtration Candida guilliermondii var. membranaefaciens

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.19 Candida guilliermondii var. membranaefaciens
-
 Y43
-
2.6.1.19 Candida guilliermondii var. membranaefaciens Y43
-
 Y43
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.19
-
 Candida guilliermondii var. membranaefaciens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.19 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate mechanism Candida guilliermondii var. membranaefaciens
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.19 52.33
-
-
 Candida guilliermondii var. membranaefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.19 4-aminobutanoate + 2-oxoglutarate best substrate Candida guilliermondii var. membranaefaciens 4-oxobutanoate + L-glutamate
-
 r
2.6.1.19 4-aminobutanoate + 2-oxoglutarate best substrate Candida guilliermondii var. membranaefaciens Y43 4-oxobutanoate + L-glutamate
-
 r
2.6.1.19 5-aminopentanoate + 2-oxoglutarate transamination at 45% the rate of 4-aminobutanoate Candida guilliermondii var. membranaefaciens L-glutamate + 5-oxopentanoate
-
 ?
2.6.1.19 5-aminopentanoate + 2-oxoglutarate transamination at 45% the rate of 4-aminobutanoate Candida guilliermondii var. membranaefaciens Y43 L-glutamate + 5-oxopentanoate
-
 ?
2.6.1.19 6-aminohexanoate + 2-oxoglutarate transamination at 27% the rate of 4-aminobutanoate Candida guilliermondii var. membranaefaciens L-glutamate + 6-oxohexanoate
-
 ?
2.6.1.19 6-aminohexanoate + 2-oxoglutarate transamination at 27% the rate of 4-aminobutanoate Candida guilliermondii var. membranaefaciens Y43 L-glutamate + 6-oxohexanoate
-
 ?
2.6.1.19 beta-alanine + 2-oxoglutarate poor substrate Candida guilliermondii var. membranaefaciens malonic semialdehyde + L-glutamate
-
 r
2.6.1.19 beta-alanine + 2-oxoglutarate poor substrate Candida guilliermondii var. membranaefaciens Y43 malonic semialdehyde + L-glutamate
-
 r
2.6.1.19 additional information no substrates are L-aminovalerate, L-norleucine, straight alpha-amino acids, diamino acids Candida guilliermondii var. membranaefaciens ?
-
 ?
2.6.1.19 additional information no substrates are L-aminovalerate, L-norleucine, straight alpha-amino acids, diamino acids Candida guilliermondii var. membranaefaciens Y43 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.19 45
-
-
 Candida guilliermondii var. membranaefaciens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.19 25 45 linear increase, above 45°C rapid decrease Candida guilliermondii var. membranaefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.19 additional information
-
 2-oxoglutarate protects against heat inactivation, better than pyridoxal phosphate Candida guilliermondii var. membranaefaciens
2.6.1.19 42
-
 up to, at least 10 min stable Candida guilliermondii var. membranaefaciens
2.6.1.19 46
-
 up to, at least 10 min stable in the presence of pyridoxal phosphate Candida guilliermondii var. membranaefaciens
2.6.1.19 50
-
 up to, at least 10 min stable in the presence of 2-oxoglutarate Candida guilliermondii var. membranaefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.19 additional information
-
 pI: 5.7 Candida guilliermondii var. membranaefaciens
2.6.1.19 7.8 8 at 35°C Candida guilliermondii var. membranaefaciens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.19 0.002
-
 Butyric acid
-
 Candida guilliermondii var. membranaefaciens
2.6.1.19 0.003
-
 propionic acid
-
 Candida guilliermondii var. membranaefaciens
2.6.1.19 0.033
-
 2-aminobutanoate
-
 Candida guilliermondii var. membranaefaciens
2.6.1.19 0.0355
-
 beta-Alanine
-
 Candida guilliermondii var. membranaefaciens
2.6.1.19 0.039
-
 alpha-alanine
-
 Candida guilliermondii var. membranaefaciens