Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schousboe, A.; Wu, J.Y.; Roberts, E.
    Purification and characterization of the 4-aminobutyrate--2,ketoglutarate transaminase from mouse brain (1973), Biochemistry, 12, 2868-2873.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.19 freeze-thawing slightly inactivates Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.19 Aminooxyacetate
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.19 0.13 0.27 2-oxoglutarate 37°C, pH 8.0 Mus musculus
2.6.1.19 1.1
-
 4-aminobutanoate 37°C, pH 8.0 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.19 mitochondrion
-
 Mus musculus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.19 109000
-
 high speed sedimentation equilibrium centrifugation Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.19 4-aminobutanoate + 2-oxoglutarate Mus musculus involved in gamma-aminobutyrate metabolism 4-oxobutanoate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.19 Mus musculus
-
 Swiss albino
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.19
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.19 brain
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.19 5
-
 37°C, pH 8.0 Mus musculus

Storage Stability

EC Number Storage Stability Organism
2.6.1.19 -20°C, protected with 2-aminoethylisothiouronium bromide hydrobromide and pyridoxal phosphate, several months Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.19 3-aminoisobutanoate + 2-oxoglutarate transamination at 55% the rate of 4-aminobutanoate Mus musculus L-glutamate + 3-oxoisobutanoate
-
 r
2.6.1.19 4-aminobutanoate + 2-oxoglutarate specific for 2-oxoglutarate Mus musculus 4-oxobutanoate + L-glutamate i.e. succinic semialdehyde + L-Glu r
2.6.1.19 4-aminobutanoate + 2-oxoglutarate involved in gamma-aminobutyrate metabolism Mus musculus 4-oxobutanoate + L-glutamate
-
 ?
2.6.1.19 5-aminopentanoate + 2-oxoglutarate transamination at 48% the rate of 4-aminobutanoate Mus musculus L-glutamate + 5-oxopentanoate
-
 ?
2.6.1.19 beta-alanine + 2-oxoglutarate effective amino group donor Mus musculus malonic semialdehyde + L-glutamate
-
 r
2.6.1.19 DL-3-hydroxy-4-aminobutanoate + 2-oxoglutarate
-
 Mus musculus L-glutamate + 3-hydroxy-4-oxobutanoate
-
 r
2.6.1.19 additional information overview Mus musculus ?
-
 ?
2.6.1.19 additional information no amino acceptors are oxaloacetate, pyruvate Mus musculus ?
-
 ?
2.6.1.19 additional information no amino acceptors are 2-oxobutyrate, phenylpyruvate, alpha-ketoadipate Mus musculus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.19 8.1
-
-
 Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.19 6.9 8.8 about half-maximal activity at pH 6.9 and 8.8 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.19 pyridoxal 5'-phosphate
-
 Mus musculus