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Literature summary extracted from

  • Ohashi, H.; Matsuhashi, M.; Matsuhashi, S.
    Thymidine diphosphate 4-acetamido-4,6-dideoxyhexoses. IV. Purification and properties of thymidine diphosphate 4-keto-6-deoxy-D-glucose transaminase from Pasteurella pseudotuberculosis (1971), J. Biol. Chem., 246, 2325-2330.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.59 Maleate stabilizing effect Yersinia pseudotuberculosis

General Stability

EC Number General Stability Organism
2.6.1.59 crude extract and partially purified enzyme are unstable, necessity of addition of pyridoxal phosphate during purification Yersinia pseudotuberculosis
2.6.1.59 maleate enhances stabilizing effect of pyridoxal phosphate Yersinia pseudotuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.59 L-cysteine strong Yersinia pseudotuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.59 0.16
-
 TDP-4-amino-4,6-dideoxy-D-galactose pH 7.0, 38°C Yersinia pseudotuberculosis
2.6.1.59 0.22
-
 2-oxoglutarate pH 7.0, 38°C Yersinia pseudotuberculosis
2.6.1.59 1
-
 TDP-4-keto-6-deoxy-D-glucose pH 7.0, 38°C Yersinia pseudotuberculosis
2.6.1.59 7.9
-
 L-glutamate pH 7.0, 38°C Yersinia pseudotuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.59 dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate Yersinia pseudotuberculosis
-
 dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
 ?
2.6.1.59 dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate Yersinia pseudotuberculosis VO
-
 dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.59 Yersinia pseudotuberculosis
-
 type VO
-
2.6.1.59 Yersinia pseudotuberculosis VO
-
 type VO
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.59 partial Yersinia pseudotuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.59 1.01
-
 pH 7.0, 38°C Yersinia pseudotuberculosis

Storage Stability

EC Number Storage Stability Organism
2.6.1.59 0°C or -15°C, purified enzyme, stable for several weeks Yersinia pseudotuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.59 dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
 Yersinia pseudotuberculosis dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
 ?
2.6.1.59 dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
 Yersinia pseudotuberculosis VO dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
 ?
2.6.1.59 additional information little activity with L-glutamine or L-alanine Yersinia pseudotuberculosis ?
-
 ?
2.6.1.59 additional information little activity with L-glutamine or L-alanine Yersinia pseudotuberculosis VO ?
-
 ?
2.6.1.59 TDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
 Yersinia pseudotuberculosis TDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
 r
2.6.1.59 TDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
 Yersinia pseudotuberculosis VO TDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.59 38
-
 assay at Yersinia pseudotuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.59 65
-
 10 min: 7% remaining activity, 72% remaining activity with addition of pyridoxal 5'-phosphate, 86% remaining activity with addition of pyridoxal 5'-phosphate and potassium maleate Yersinia pseudotuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.59 7
-
-
 Yersinia pseudotuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.59 pyridoxal 5'-phosphate absolute requirement, a pyridoxal phosphate protein Yersinia pseudotuberculosis