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Literature summary extracted from

  • Minatogawa, Y.; Noguchi, T.; Kido, R.
    Species distribution and properties of hepatic phenylalanine (histidine):pyruvate aminotransferase (1977), Hoppe-Seyler's Z. Physiol. Chem., 358, 59-67.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.58 pyridoxal 5'-phosphate
-
 Mus musculus

General Stability

EC Number General Stability Organism
2.6.1.58 freezing and thawing, loss of most of activity Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.58 histidine 10 mM: 56% inhibition with L-phenylalanine and pyruvate as substrates Mus musculus
2.6.1.58 hydroxylamine 0.5 mM: 63% inhibition Mus musculus
2.6.1.58 Isonicotinic acid hydrazide 2 mM: 27% inhibition Mus musculus
2.6.1.58 KCN 1 mM: 22% inhibition Mus musculus
2.6.1.58 L-methionine 10 mM: competitive inhibitor Mus musculus
2.6.1.58 p-chloromercuribenzoate 0.25 mM: 48% inhibition, reactivation by addition of 2-mercaptoethanol Mus musculus
2.6.1.58 Semicarbazide 1 mM: 12% inhibition Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.58 6.9
-
 histidine cosubstrate pyruvate, pH 8.9, 37°C Mus musculus
2.6.1.58 21
-
 pyruvate cosubstrate L-histidine, pH 8.9, 37°C Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.58 cytosol mitochondrial fraction: 70% of enzymatic activity, cytosolic fraction: 12% Mus musculus 5829
-
2.6.1.58 mitochondrion mitochondrial fraction: 70% of enzymatic activity, cytosolic fraction: 12% Mus musculus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.58 71000 81000 gel filtration Mus musculus
2.6.1.58 75000 85000 sucrose density gradient centrifugation Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.58 L-phenylalanine + pyruvate Mus musculus
-
 phenylpyruvate + L-alanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.58 Canis lupus familiaris
-
-
-
2.6.1.58 Cavia porcellus
-
-
-
2.6.1.58 Gallus gallus
-
-
-
2.6.1.58 Homo sapiens
-
-
-
2.6.1.58 Mus musculus
-
 injection of glucagon elevates activity
-
2.6.1.58 Oryctolagus cuniculus
-
-
-
2.6.1.58 Rattus norvegicus
-
 injection of glucagon elevates activity
-
2.6.1.58 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.58 partial, isoelectric focusing of the purified enzyme results in detection of 4 forms with different pI, all forms are nearly identical with respect to physical and enzymatic properties, all forms are responsive to glucagon Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.58 liver
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.58 13.8
-
 L-histidine with pyruvate, pH 8.9, 37°C Mus musculus

Storage Stability

EC Number Storage Stability Organism
2.6.1.58 4°C, purified enzyme in 0.05 mM potassium phosphate buffer, pH 7.5, rapid inactivation Mus musculus
2.6.1.58 4°C, purified enzyme in 50 mM potassium phosphate buffer, pH 7.5, stable for at least 2 weeks Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.58 L-glutamine + phenylpyruvate
-
 Mus musculus 5-amino-2,5-dioxopentanoic acid + L-phenylalanine
-
 ?
2.6.1.58 L-histidine + pyruvate about 50% of activity compared to L-phenylalanine Mus musculus 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
-
 ?
2.6.1.58 L-methionine + phenylpyruvate
-
 Mus musculus 4-methylsulfanyl-2-oxobutanoate + L-phenylalanine
-
 ?
2.6.1.58 L-phenylalanine + pyruvate
-
 Mus musculus phenylpyruvate + L-alanine
-
 ?
2.6.1.58 L-phenylalanine + pyruvate highest activity Mus musculus phenylpyruvate + L-alanine
-
 ?
2.6.1.58 L-serine + phenylpyruvate
-
 Mus musculus 3-hydroxy-2-oxopropanoate + L-phenylalanine
-
 ?
2.6.1.58 L-tyrosine + pyruvate about 27% of activity compared to L-phenylalanine Mus musculus 3-(4-hydroxyphenyl)-2-oxopropanoate + L-alanine
-
 ?
2.6.1.58 additional information oxaloacetate and hydroxypyruvate may replace pyruvate as amino acceptor at lower rates, little or no activity with 2-oxoglutarate Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.58 dimer 2 * 38000-42000, SDS-PAGE Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.58 37
-
 assay at Gallus gallus
2.6.1.58 37
-
 assay at Cavia porcellus
2.6.1.58 37
-
 assay at Mus musculus
2.6.1.58 37
-
 assay at Homo sapiens
2.6.1.58 37
-
 assay at Rattus norvegicus
2.6.1.58 37
-
 assay at Sus scrofa
2.6.1.58 37
-
 assay at Oryctolagus cuniculus
2.6.1.58 37
-
 assay at Canis lupus familiaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.58 80
-
 1 h, in 15 mM potassium phosphate buffer, pH 7.5: stable Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.58 9
-
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.58 pyridoxal 5'-phosphate
-
 Mus musculus