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Literature summary extracted from

  • Smith, R.A.; Theisen, M.C.
    Phosphoramidate-hexose transphosphorylase (1966), Methods Enzymol., 9, 403-407.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.62 F- 1.5 mM, at pH 8.7 8% inhibition, at pH 6.5 90% inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.62 12.5
-
 glucose 37°C, pH 8.7 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.62 phosphoramidate + D-glucose Escherichia coli phosphoramidate, i.e. PNH2, is delivered by KO3PNH3 D-glucose 1-phosphate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.62 Escherichia coli
-
 Crookes strain, ATCC 8739
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.62
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.62 22.7
-
 37°C, pH 8.7 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.1.62 4°C or -15°C, 6 years stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.62 phosphoramidate + 2-deoxy-D-glucose
-
 Escherichia coli 2-deoxy-D-glucose 1-phosphate + NH3
-
 ?
2.7.1.62 phosphoramidate + 3-O-methylglucose
-
 Escherichia coli 3-O-methylglucose 1-phosphate + NH3
-
 ?
2.7.1.62 phosphoramidate + D-glucose glucose is best substrate, phosphoramidate, i.e. PNH2, is delivered by KO3PNH3 Escherichia coli D-glucose 1-phosphate + NH3
-
 ?
2.7.1.62 phosphoramidate + D-glucose phosphoramidate, i.e. PNH2, is delivered by KO3PNH3 Escherichia coli D-glucose 1-phosphate + NH3
-
 ?
2.7.1.62 phosphoramidate + fructose
-
 Escherichia coli fructose 1-phosphate + NH3
-
 ?
2.7.1.62 phosphoramidate + glucosamine
-
 Escherichia coli glucosamine 1-phosphate + NH3
-
 ?
2.7.1.62 phosphoramidate + mannose
-
 Escherichia coli mannose 1-phosphate + NH3
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.62 37
-
 assay at Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.62 55
-
 pH 7.5, more than 30 min, no loss of activity Escherichia coli
2.7.1.62 100
-
 10 min, about 60% loss of activity Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.62 8.7
-
 assay at Escherichia coli