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Literature summary extracted from

  • Wright, G.D.; Ladak, P.
    Overexpression and characterization of the chromosomal aminoglycoside 6'-N-acetyltransferase from Enterococcus faecium (1997), Antimicrob. Agents Chemother., 41, 956-960.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.82 expression in Escherichia coli Enterococcus faecium

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.82 F171L
-
 Enterococcus faecium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.82 butyryl-CoA
-
 Enterococcus faecium
2.3.1.82 dibekacin
-
 Enterococcus faecium
2.3.1.82 kanamycin A
-
 Enterococcus faecium
2.3.1.82 kanamycin B
-
 Enterococcus faecium
2.3.1.82 netilmicin
-
 Enterococcus faecium
2.3.1.82 sisomycin
-
 Enterococcus faecium
2.3.1.82 tobramycin
-
 Enterococcus faecium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.82 0.00531
-
 neomycin C pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.00582
-
 neamine pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.00638
-
 netilmicin pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.00908
-
 ribostamycin pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.0117
-
 sisomicin pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.0131
-
 amikacin pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.014
-
 butirosin pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.0189
-
 kanamycin B pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.0195
-
 propionyl-CoA pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.0199
-
 kanamycin A pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.022
-
 tobramycin pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.0223
-
 gentamicin B pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.0235
-
 acetyl-CoA pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.036
-
 dibekacin pH 6.0, 37°C Enterococcus faecium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.82 additional information Enterococcus faecium involved in resistance to antibiotics ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.82 Enterococcus faecium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.82
-
 Enterococcus faecium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.82 acetyl-CoA + amikacin
-
 Enterococcus faecium CoA + N6'-acetylamikacin
-
 ?
2.3.1.82 acetyl-CoA + butirosin
-
 Enterococcus faecium CoA + N6'-acetylbutirosin
-
 ?
2.3.1.82 acetyl-CoA + dibekacin
-
 Enterococcus faecium CoA + N6'-acetyldibekacin
-
 ?
2.3.1.82 acetyl-CoA + gentamicin B
-
 Enterococcus faecium CoA + N6'-acetylgentamicin B
-
 ?
2.3.1.82 acetyl-CoA + kanamycin A
-
 Enterococcus faecium CoA + N6'-acetylkanamycin A
-
 ?
2.3.1.82 acetyl-CoA + kanamycin B
-
 Enterococcus faecium CoA + N6'-acetylkanamycin B
-
 ?
2.3.1.82 acetyl-CoA + neamine
-
 Enterococcus faecium CoA + 6'-N-acetylneamine
-
 ?
2.3.1.82 acetyl-CoA + neomycin C
-
 Enterococcus faecium CoA + N6'-acetylneomycin C
-
 ?
2.3.1.82 acetyl-CoA + netilmicin
-
 Enterococcus faecium CoA + N6'-acetylnetilmicin
-
 ?
2.3.1.82 acetyl-CoA + ribostamycin
-
 Enterococcus faecium CoA + N6'-acetylribostamycin
-
 ?
2.3.1.82 acetyl-CoA + sisomicin
-
 Enterococcus faecium CoA + N6'-acetylsisomicin
-
 ?
2.3.1.82 acetyl-CoA + tobramycin
-
 Enterococcus faecium CoA + N6'-acetyltobramycin
-
 ?
2.3.1.82 additional information acetyl-CoA and propionyl-CoA are comparable substrates, but butyryl-CoA is not Enterococcus faecium ?
-
 ?
2.3.1.82 additional information involved in resistance to antibiotics Enterococcus faecium ?
-
 ?
2.3.1.82 propionyl-CoA + kanamycin A
-
 Enterococcus faecium CoA + N6'-propionylkanamycin A
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.82 0.106
-
 amikacin pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.205
-
 neomycin C pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.317
-
 propionyl-CoA pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.338
-
 ribostamycin pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.37
-
 sisomicin pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.388
-
 gentamicin B pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.403
-
 acetyl-CoA pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.419
-
 neamine pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.466
-
 butirosin pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.512
-
 netilmicin pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.632
-
 dibekacin pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.816
-
 kanamycin A pH 6.0, 37°C Enterococcus faecium
2.3.1.82 1.08
-
 kanamycin B pH 6.0, 37°C Enterococcus faecium
2.3.1.82 1.11
-
 tobramycin pH 6.0, 37°C Enterococcus faecium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.82 0.059
-
 tobramycin pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.063
-
 sisomicin pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.072
-
 dibekacin pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.117
-
 kanamycin B pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.142
-
 netilmicin pH 6.0, 37°C Enterococcus faecium
2.3.1.82 0.196
-
 kanamycin A pH 6.0, 37°C Enterococcus faecium