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Literature summary extracted from

  • Meyer, J.F.; Wiedemann, B.
    Characterization of aminoglycoside 6-N-acetyltransferases [AAC(6)] from gram-negative bacteria and Streptomyces kanamyceticus (1985), J. Antimicrob. Chemother., 15, 271-282.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.82 Pseudomonas aeruginosa 141, gene transfer via plasmid pBP30 to Escherichia coli Hb101 Escherichia coli
2.3.1.82 Pseudomonas aeruginosa 141, gene transfer via plasmid pBP30 to Escherichia coli Hb101 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.82 0.001
-
 kanamycin A pH 7.5 Serratia marcescens
2.3.1.82 0.001
-
 kanamycin A pH 6.0 Pseudomonas aeruginosa
2.3.1.82 0.005
-
 kanamycin A pH 6.0 Pseudomonas aeruginosa
2.3.1.82 0.006
-
 kanamycin A pH 7.5 Serratia marcescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.82 55000
-
 x * 55000, strain GN315, SDS-PAGE Serratia marcescens
2.3.1.82 57000
-
 x * 57000, SDS-PAGE Serratia marcescens
2.3.1.82 59000
-
 x * 59000, strain 141, SDS-PAGE Pseudomonas aeruginosa
2.3.1.82 75000
-
 x * 75000, SDS-PAGE Streptomyces kanamyceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.82 additional information Escherichia coli involved in resistance to antibiotics ?
-
 ?
2.3.1.82 additional information Pseudomonas aeruginosa involved in resistance to antibiotics ?
-
 ?
2.3.1.82 additional information Serratia marcescens involved in resistance to antibiotics ?
-
 ?
2.3.1.82 additional information Streptomyces kanamyceticus involved in resistance to antibiotics ?
-
 ?
2.3.1.82 additional information Escherichia coli NR79 involved in resistance to antibiotics ?
-
 ?
2.3.1.82 additional information Serratia marcescens VU12944/77 involved in resistance to antibiotics ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.82 Escherichia coli
-
 NR79
-
2.3.1.82 Escherichia coli NR79
-
 NR79
-
2.3.1.82 Pseudomonas aeruginosa
-
 strains GN315 and 141
-
2.3.1.82 Serratia marcescens
-
-
-
2.3.1.82 Serratia marcescens VU12944/77
-
-
-
2.3.1.82 Streptomyces kanamyceticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.82
-
 Escherichia coli
2.3.1.82
-
 Serratia marcescens
2.3.1.82
-
 Streptomyces kanamyceticus
2.3.1.82 strain GN315 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.82 acetyl-CoA + amikacin
-
 Escherichia coli CoA + N6'-acetylamikacin
-
 ?
2.3.1.82 acetyl-CoA + amikacin
-
 Pseudomonas aeruginosa CoA + N6'-acetylamikacin
-
 ?
2.3.1.82 acetyl-CoA + amikacin
-
 Serratia marcescens CoA + N6'-acetylamikacin
-
 ?
2.3.1.82 acetyl-CoA + amikacin
-
 Streptomyces kanamyceticus CoA + N6'-acetylamikacin
-
 ?
2.3.1.82 acetyl-CoA + dibekacin
-
 Escherichia coli CoA + N6'-acetyldibekacin
-
 ?
2.3.1.82 acetyl-CoA + dibekacin
-
 Pseudomonas aeruginosa CoA + N6'-acetyldibekacin
-
 ?
2.3.1.82 acetyl-CoA + dibekacin
-
 Serratia marcescens CoA + N6'-acetyldibekacin
-
 ?
2.3.1.82 acetyl-CoA + dibekacin
-
 Streptomyces kanamyceticus CoA + N6'-acetyldibekacin
-
 ?
2.3.1.82 acetyl-CoA + dibekacin
-
 Escherichia coli NR79 CoA + N6'-acetyldibekacin
-
 ?
2.3.1.82 acetyl-CoA + dibekacin
-
 Serratia marcescens VU12944/77 CoA + N6'-acetyldibekacin
-
 ?
2.3.1.82 acetyl-CoA + gentamicin C1a
-
 Escherichia coli CoA + N6'-acetylgentamicin C1a
-
 ?
2.3.1.82 acetyl-CoA + gentamicin C1a
-
 Pseudomonas aeruginosa CoA + N6'-acetylgentamicin C1a
-
 ?
2.3.1.82 acetyl-CoA + gentamicin C1a
-
 Serratia marcescens CoA + N6'-acetylgentamicin C1a
-
 ?
2.3.1.82 acetyl-CoA + gentamicin C1a
-
 Streptomyces kanamyceticus CoA + N6'-acetylgentamicin C1a
-
 ?
2.3.1.82 acetyl-CoA + gentamicin C1a
-
 Escherichia coli NR79 CoA + N6'-acetylgentamicin C1a
-
 ?
2.3.1.82 acetyl-CoA + gentamicin C1a
-
 Serratia marcescens VU12944/77 CoA + N6'-acetylgentamicin C1a
-
 ?
2.3.1.82 acetyl-CoA + kanamycin A
-
 Escherichia coli CoA + N6'-acetylkanamycin A
-
 ?
2.3.1.82 acetyl-CoA + kanamycin A
-
 Pseudomonas aeruginosa CoA + N6'-acetylkanamycin A
-
 ?
2.3.1.82 acetyl-CoA + kanamycin A
-
 Serratia marcescens CoA + N6'-acetylkanamycin A
-
 ?
2.3.1.82 acetyl-CoA + kanamycin A
-
 Streptomyces kanamyceticus CoA + N6'-acetylkanamycin A
-
 ?
2.3.1.82 acetyl-CoA + kanamycin A
-
 Escherichia coli NR79 CoA + N6'-acetylkanamycin A
-
 ?
2.3.1.82 acetyl-CoA + kanamycin A
-
 Serratia marcescens VU12944/77 CoA + N6'-acetylkanamycin A
-
 ?
2.3.1.82 acetyl-CoA + tobramycin
-
 Escherichia coli CoA + N6'-acetyltobramycin
-
 ?
2.3.1.82 acetyl-CoA + tobramycin
-
 Serratia marcescens CoA + N6'-acetyltobramycin
-
 ?
2.3.1.82 acetyl-CoA + tobramycin
-
 Streptomyces kanamyceticus CoA + N6'-acetyltobramycin
-
 ?
2.3.1.82 acetyl-CoA + tobramycin
-
 Escherichia coli NR79 CoA + N6'-acetyltobramycin
-
 ?
2.3.1.82 acetyl-CoA + tobramycin
-
 Serratia marcescens VU12944/77 CoA + N6'-acetyltobramycin
-
 ?
2.3.1.82 additional information involved in resistance to antibiotics Escherichia coli ?
-
 ?
2.3.1.82 additional information involved in resistance to antibiotics Pseudomonas aeruginosa ?
-
 ?
2.3.1.82 additional information involved in resistance to antibiotics Serratia marcescens ?
-
 ?
2.3.1.82 additional information involved in resistance to antibiotics Streptomyces kanamyceticus ?
-
 ?
2.3.1.82 additional information involved in resistance to antibiotics Escherichia coli NR79 ?
-
 ?
2.3.1.82 additional information involved in resistance to antibiotics Serratia marcescens VU12944/77 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.82 ? x * 57000, SDS-PAGE Serratia marcescens
2.3.1.82 ? x * 75000, SDS-PAGE Streptomyces kanamyceticus
2.3.1.82 ? x * 55000, strain GN315, SDS-PAGE Serratia marcescens
2.3.1.82 ? x * 59000, strain 141, SDS-PAGE Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.82 6
-
-
 Escherichia coli
2.3.1.82 6
-
-
 Pseudomonas aeruginosa
2.3.1.82 6.5
-
-
 Pseudomonas aeruginosa
2.3.1.82 7
-
-
 Streptomyces kanamyceticus
2.3.1.82 7.5
-
-
 Serratia marcescens