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Literature summary extracted from

  • Freischutz, B.; Saito, M.; Rahmann, H.; Yu, R.K.
    Activities of five different sialyltransferases in fish and rat brains (1994), J. Neurochem., 62, 1965-1973.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.3.1 membrane
-
 Rattus norvegicus 16020
-
2.4.3.1 membrane
-
 Oreochromis mossambicus 16020
-
2.4.3.8 membrane
-
 Rattus norvegicus 16020
-
2.4.3.8 membrane
-
 Oreochromis mossambicus 16020
-
2.4.3.9 membrane
-
 Rattus norvegicus 16020
-
2.4.3.9 membrane
-
 Oreochromis mossambicus 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Oreochromis mossambicus
-
 cichlid fish
-
2.4.3.1 Rattus norvegicus
-
 Sprague-Dawley
-
2.4.3.1 Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-
2.4.3.2 cichlidae
-
-
-
2.4.3.2 Rattus norvegicus
-
-
-
2.4.3.8 Oreochromis mossambicus
-
 cichlid fish
-
2.4.3.8 Rattus norvegicus
-
 Sprague-Dawley
-
2.4.3.8 Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-
2.4.3.9 Oreochromis mossambicus
-
 cichlid fish
-
2.4.3.9 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.1 homogenized, centrifuged, resuspended in 25 mM-cacodylate buffer pH 6.5 containing 0.15% Triton X-100, 75 mM NaCl and 10 mM MnCl2 Rattus norvegicus
2.4.3.1 homogenized, centrifuged, resuspended in 25 mM-cacodylate buffer pH 6.5 containing 0.15% Triton X-100, 75 mM NaCl and 10 mM MnCl2 Oreochromis mossambicus
2.4.3.8 homogenized, centrifuged, resuspended in 25mM-cacodylate buffer pH 6.5 containing 0.15% Triton X-100, 75mM NaCl and 10 mM MnCl2 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.1 brain
-
 Rattus norvegicus
-
2.4.3.1 brain
-
 Oreochromis mossambicus
-
2.4.3.2 brain
-
 Rattus norvegicus
-
2.4.3.2 brain
-
 cichlidae
-
2.4.3.8 brain
-
 Rattus norvegicus
-
2.4.3.8 brain
-
 Oreochromis mossambicus
-
2.4.3.9 brain
-
 Rattus norvegicus
-
2.4.3.9 brain
-
 Oreochromis mossambicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.3.2 0.00000186
-
-
 cichlidae
2.4.3.2 0.0000033
-
-
 cichlidae
2.4.3.9 0.0000027
-
 sialyltransferase-1 activity in membrane fraction from brain Oreochromis mossambicus
2.4.3.9 0.0000064
-
 sialyltransferase-1 activity in membrane fraction from brain Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. ganglioside GM3 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 Oreochromis mossambicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. ganglioside GM3 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 Rattus norvegicus Sprague-Dawley CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. ganglioside GM3 ?
2.4.3.2 CMP-N-acetyl-beta-neuraminate + ganglioside GM1a
-
 Rattus norvegicus CMP + ganglioside GD1a i.e. ganglioside GD1a ?
2.4.3.2 CMP-N-acetyl-beta-neuraminate + ganglioside GM1a
-
 cichlidae CMP + ganglioside GD1a i.e. ganglioside GD1a ?
2.4.3.2 CMP-N-acetyl-beta-neuraminate + ganglioside GM1a
-
 Rattus norvegicus Sprague-Dawley CMP + ganglioside GD1a i.e. ganglioside GD1a ?
2.4.3.9 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 Rattus norvegicus CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. ganglioside GM3 ?
2.4.3.9 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 Oreochromis mossambicus CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. ganglioside GM3 ?
2.4.3.9 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 Rattus norvegicus Sprague-Dawley CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. ganglioside GM3 ?