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Literature summary extracted from

  • Higashi, H.; Basu, M.; Basu, S.
    Biosynthesis in vitro of disialosylneolactotetraosylceramide by a solubilized sialyltransferase from embryonic chicken brain (1985), J. Biol. Chem., 260, 824-828.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.3.8 Nonidet P-40 activation Gallus gallus
2.4.3.8 Triton CF-54
-
 Gallus gallus
2.4.3.8 Triton X-100
-
 Gallus gallus
2.4.3.8 Zwittergent 3-10
-
 Gallus gallus
2.4.3.8 Zwittergent 3-14
-
 Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.3.8 Ganglioside LM1 at higher concentrations, substrate inhibition Gallus gallus
2.4.3.8 N-ethylmaleimide
-
 Gallus gallus
2.4.3.9 additional information not inhibited by N-ethylmaleimide Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.8 0.063
-
 Ganglioside LM1 soluble enzyme preparation Gallus gallus
2.4.3.8 0.07
-
 CMP-N-acetylneuraminate
-
 Gallus gallus
2.4.3.8 0.078
-
 ganglioside GM3
-
 Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.3.8 membrane
-
 Gallus gallus 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.8 Gallus gallus
-
-
-
2.4.3.9 Gallus gallus
-
 7-11 days old embryo
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.8 brain
-
 Gallus gallus
-
2.4.3.9 brain
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.8 CMP-N-acetylneuraminate + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide i.e. sialosylneolactotetraosylceramide or ganglioside LM1 Gallus gallus CMP + alpha-N-acetylneuraminyl-2,8-alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide i.e. disialosylneolactotetraosylceramide or ganglioside LD1c ?
2.4.3.8 CMP-N-acetylneuraminate + ganglioside GM3
-
 Gallus gallus CMP + ganglioside GD3
-
 ?
2.4.3.8 CMP-N-acetylneuraminate + ganglioside LM1
-
 Gallus gallus CMP + ganglioside LD1c
-
 ?
2.4.3.8 CMP-N-acetylneuraminate + N-alpha-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 Gallus gallus CMP + alpha-N-acetylneuraminyl-2,8-alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. disialosyllactosylceramide or ganglioside GD3 ?
2.4.3.9 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 Gallus gallus CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. ganglioside GM3 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.3.8 37
-
 assay at Gallus gallus