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Literature summary extracted from

  • Debussche, L.; Couder, M.; Thibaut, D.; Cameron, B.; Crouzet, J.; Blanche, F.
    Purification and partial characterization of Cob(I)alamin adenosyltransferase from Pseudomonas denitrificans (1991), J. Bacteriol., 173, 6300-6302.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.5.1.17 DTT stabilizes during purification and storage, removal leads to complete loss of activity Pseudomonas denitrificans (nom. rej.)

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.17 hydrogenobyrinic acid a,c-diamide cobalt-free analogue of cobyrinic acid a,c-diamide Pseudomonas denitrificans (nom. rej.)
2.5.1.17 additional information no inhibition by cobyrinic acid, hydrogenobyrinic acid Pseudomonas denitrificans (nom. rej.)
2.5.1.17 tripolyphosphate
-
 Pseudomonas denitrificans (nom. rej.)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.17 soluble
-
 Pseudomonas denitrificans (nom. rej.)
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.17 Mn2+
-
 Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.17 28000
-
 2 * 28000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
2.5.1.17 56000
-
 HPLC gel filtration Pseudomonas denitrificans (nom. rej.)
2.5.1.17 70000
-
 PAGE Pseudomonas denitrificans (nom. rej.)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.17 ATP + cob(I)alamin Pseudomonas denitrificans (nom. rej.)
-
 triphosphate + coenzyme B12
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.17 Pseudomonas denitrificans (nom. rej.) P29930 recombinant strain SC510 Rifr containing plasmid pXL227, this DNA seuqence encodes for several enzymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.17 centrifugation, Mono Q chromatography and gel filtration Pseudomonas denitrificans (nom. rej.)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.17 0.089
-
 pH 8.0, 30°C Pseudomonas denitrificans (nom. rej.)
2.5.1.17 0.089
-
 in the presence of cobalamin as substrate Pseudomonas denitrificans (nom. rej.)
2.5.1.17 0.13
-
 pH 8.0, 30°C Pseudomonas denitrificans (nom. rej.)
2.5.1.17 0.13
-
 in the presence of cobyrinic acid a,c-diamide as substrate Pseudomonas denitrificans (nom. rej.)
2.5.1.17 0.14
-
 pH 8.0, 30°C Pseudomonas denitrificans (nom. rej.)
2.5.1.17 0.14
-
 in the presence of cobyric acid as substrate Pseudomonas denitrificans (nom. rej.)
2.5.1.17 0.18
-
 pH 8.0, 30°C Pseudomonas denitrificans (nom. rej.)
2.5.1.17 0.18
-
 in the presence of cobinamide as substrate Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.17 ATP + cob(I)alamin
-
 Pseudomonas denitrificans (nom. rej.) triphosphate + coenzyme B12
-
 ?
2.5.1.17 ATP + cob(I)alamin
-
 Pseudomonas denitrificans (nom. rej.) triphosphate + coenzyme B12 adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 ?
2.5.1.17 cob(I)inamide + ATP
-
 Pseudomonas denitrificans (nom. rej.) 5'-deoxy-5'-adenosyl-cob(I)inamide + polyphosphate
-
 ?
2.5.1.17 cob(I)yric acid + ATP
-
 Pseudomonas denitrificans (nom. rej.) 5'-deoxy-5'-adenosyl-cob(I)yric acid + polyphosphate
-
 ?
2.5.1.17 cob(I)yrinic acid a,c-diamide + ATP
-
 Pseudomonas denitrificans (nom. rej.) 5'-deoxy-5'-adenosyl-cob(I)yrinic acid a,c-diamide + polyphosphate
-
 ?
2.5.1.17 additional information cobyrinic acid is not a substrate Pseudomonas denitrificans (nom. rej.) ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.17 dimer 2 * 28000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.17 30
-
 assay at Pseudomonas denitrificans (nom. rej.)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.17 8
-
 assay at Pseudomonas denitrificans (nom. rej.)