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Literature summary extracted from

  • Beck, W.S.
    Ribosome-associated vitamin B12s adenosylating enzyme of Lactobacillus leichmannii (1980), Methods Enzymol., 67, 41-56.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.17 2-mercaptoethanol stabilizes during purification Clostridium tetanomorphum
2.5.1.17 2-mercaptoethanol stabilizes during purification Lactobacillus leichmannii
2.5.1.17 repeated freeze-thawing inactivates Clostridium tetanomorphum
2.5.1.17 repeated freeze-thawing inactivates Lactobacillus leichmannii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.17 Ca2+
-
 Lactobacillus leichmannii
2.5.1.17 Cd2+
-
 Lactobacillus leichmannii
2.5.1.17 diphosphate
-
 Clostridium tetanomorphum
2.5.1.17 diphosphate
-
 Lactobacillus leichmannii
2.5.1.17 Mn2+ above 10 mM Lactobacillus leichmannii
2.5.1.17 tripolyphosphate
-
 Clostridium tetanomorphum
2.5.1.17 tripolyphosphate
-
 Lactobacillus leichmannii
2.5.1.17 Zn2+
-
 Lactobacillus leichmannii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.17 1.3
-
 ATP pH 8.0, 37°C Lactobacillus leichmannii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.17 additional information subcellular distribution Lactobacillus leichmannii
-
-
2.5.1.17 ribosome
-
 Lactobacillus delbrueckii 5840
-
2.5.1.17 ribosome
-
 Lactobacillus leichmannii 5840
-
2.5.1.17 soluble
-
 Clostridium tetanomorphum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.17 Cs+ activation Lactobacillus leichmannii
2.5.1.17 K+
-
 Clostridium tetanomorphum
2.5.1.17 K+ activation Lactobacillus leichmannii
2.5.1.17 K+ may be acting upon a step in the reduction of hydroxocobamide Propionibacterium freudenreichii subsp. shermanii
2.5.1.17 Mg2+
-
 Propionibacterium freudenreichii subsp. shermanii
2.5.1.17 Mg2+ activation, less effective than Mn2+ Clostridium tetanomorphum
2.5.1.17 Mg2+ activation, less effective than Mn2+ Lactobacillus leichmannii
2.5.1.17 Mn2+
-
 Clostridium tetanomorphum
2.5.1.17 Mn2+
-
 Lactobacillus leichmannii
2.5.1.17 Na+ activation Lactobacillus leichmannii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.17 ATP + cob(I)alamin Propionibacterium freudenreichii subsp. shermanii
-
 triphosphate + coenzyme B12
-
 ?
2.5.1.17 ATP + cob(I)alamin Lactobacillus delbrueckii
-
 triphosphate + coenzyme B12
-
 ?
2.5.1.17 ATP + cob(I)alamin Clostridium tetanomorphum
-
 triphosphate + coenzyme B12
-
 ?
2.5.1.17 ATP + cob(I)alamin Lactobacillus leichmannii
-
 triphosphate + coenzyme B12
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.17 Clostridium tetanomorphum
-
-
-
2.5.1.17 Lactobacillus delbrueckii
-
-
-
2.5.1.17 Lactobacillus leichmannii
-
-
-
2.5.1.17 Propionibacterium freudenreichii subsp. shermanii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.17 partial purification of the enzyme is achieved from ribosomal fraction by salt extraction and ammonium sulfate fractionation, followed by two chromatographic steps Lactobacillus leichmannii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.17 0.000125
-
 activity measured using washed ribosomes Lactobacillus leichmannii
2.5.1.17 0.000125
-
 pH 8.0, 37°C Lactobacillus leichmannii

Storage Stability

EC Number Storage Stability Organism
2.5.1.17 -20°C, more than 50% loss of activity within 1 week, with 50% glycerol only 20% loss of activity Lactobacillus leichmannii
2.5.1.17 2-mercaptoethanol stabilizes during storage Clostridium tetanomorphum
2.5.1.17 2-mercaptoethanol stabilizes during storage Lactobacillus leichmannii
2.5.1.17 DTT stabilizes during storage Lactobacillus leichmannii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.17 ATP + cob(I)alamin
-
 Propionibacterium freudenreichii subsp. shermanii triphosphate + coenzyme B12
-
 ?
2.5.1.17 ATP + cob(I)alamin
-
 Lactobacillus delbrueckii triphosphate + coenzyme B12
-
 ?
2.5.1.17 ATP + cob(I)alamin
-
 Clostridium tetanomorphum triphosphate + coenzyme B12
-
 ?
2.5.1.17 ATP + cob(I)alamin
-
 Lactobacillus leichmannii triphosphate + coenzyme B12
-
 ?
2.5.1.17 ATP + cob(I)alamin
-
 Propionibacterium freudenreichii subsp. shermanii triphosphate + coenzyme B12 adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 ?
2.5.1.17 ATP + cob(I)alamin
-
 Lactobacillus delbrueckii triphosphate + coenzyme B12 adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 ?
2.5.1.17 ATP + cob(I)alamin
-
 Clostridium tetanomorphum triphosphate + coenzyme B12 adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 ?
2.5.1.17 ATP + cob(I)alamin
-
 Lactobacillus leichmannii triphosphate + coenzyme B12 adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 ?
2.5.1.17 additional information
-
 Propionibacterium freudenreichii subsp. shermanii ?
-
 ?
2.5.1.17 additional information
-
 Clostridium tetanomorphum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.17 37
-
 assay at Clostridium tetanomorphum
2.5.1.17 37
-
 assay at Lactobacillus leichmannii