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Literature summary extracted from

  • Vitols, E.; Walker, G.A.; Huennekens, F.M.
    Enzymatic conversion of vitamin B-12s to a cobamide coenzyme, alpha-(5,6-dimethylbenzimidazolyl)deoxyadenosylcobamide (adenosyl-B-12) (1966), J. Biol. Chem., 241, 1455-1461.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.17 2-mercaptoethanol stabilizes during purification Clostridium tetanomorphum
2.5.1.17 repeated freeze-thawing inactivates Clostridium tetanomorphum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.17 diphosphate
-
 Clostridium tetanomorphum
2.5.1.17 EDTA
-
 Clostridium tetanomorphum
2.5.1.17 additional information no inhibition by phosphate Clostridium tetanomorphum
2.5.1.17 Trimetaphosphate
-
 Clostridium tetanomorphum
2.5.1.17 tripolyphosphate
-
 Clostridium tetanomorphum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.17 0.01
-
 cyanocob(I)alamin pH 8.0, 37°C, i.e. vitamin B12, reduced form Clostridium tetanomorphum
2.5.1.17 0.016
-
 ATP pH 8.0, 37°C Clostridium tetanomorphum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.17 soluble
-
 Clostridium tetanomorphum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.17 Co2+ activation, less effective than Mn2+ Clostridium tetanomorphum
2.5.1.17 Mg2+ activation, less effective than Mn2+ Clostridium tetanomorphum
2.5.1.17 Mn2+
-
 Clostridium tetanomorphum
2.5.1.17 additional information no activation by Ca2+ or Cd2+ Clostridium tetanomorphum
2.5.1.17 Zn2+ activation, less effective than Mn2+ Clostridium tetanomorphum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.17 ATP + cob(I)alamin Clostridium tetanomorphum involved in vitamin B12 metabolism triphosphate + coenzyme B12
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.17 Clostridium tetanomorphum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.17 protamine sulfate, heat treatment and chromatography on Sephadex G-25 and DEAE-cellulose Clostridium tetanomorphum

Storage Stability

EC Number Storage Stability Organism
2.5.1.17 -20°C, lyophilized, at least 1 week Clostridium tetanomorphum
2.5.1.17 2-mercaptoethanol stabilizes during storage Clostridium tetanomorphum
2.5.1.17 4°C, about 1 month Clostridium tetanomorphum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.17 ATP + cob(I)alamin transfers the adenosyl-group of ATP to the reduced cobalt atom of the cobalamin molecule Clostridium tetanomorphum triphosphate + coenzyme B12 adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 ?
2.5.1.17 ATP + cob(I)alamin involved in vitamin B12 metabolism Clostridium tetanomorphum triphosphate + coenzyme B12
-
 ?
2.5.1.17 cyanocob(I)alamin + ATP
-
 Clostridium tetanomorphum tripolyphosphate + alpha-(5,6-dimethylbenzimidazolyl)deoxyadenosylcobamide
-
 ?
2.5.1.17 additional information S-adenosylmethionine, vitamin B12r or B12a, AMP, ADP are no substrates Clostridium tetanomorphum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.17 37
-
 assay at Clostridium tetanomorphum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.17 60
-
 no appreciable loss of activity after 10 min Clostridium tetanomorphum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.17 8
-
 phosphate or Tris buffer Clostridium tetanomorphum