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Literature summary extracted from

  • Takahashi, I.; Ojima, N.; Ogura, K.; Seto, S.
    Purification and characterization of dimethylallyl pyrophosphate: aspulvinone dimethylallyltransferase from Aspergillus terreus (1978), Biochemistry, 17, 2696-2702.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.35 addition of 2-mercaptoethanol does not affect stability Aspergillus terreus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.35 Aspulvinone E dimethyl ether
-
 Aspergillus terreus
2.5.1.35 Bromophenol blue
-
 Aspergillus terreus
2.5.1.35 diphosphate noncompetitive against aspulvinone, mixed type against dimethylallyl diphosphate Aspergillus terreus
2.5.1.35 isopentenyl diphosphate
-
 Aspergillus terreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.35 0.0077
-
 aspulvinone G
-
 Aspergillus terreus
2.5.1.35 0.0137
-
 aspulvinone E
-
 Aspergillus terreus
2.5.1.35 0.04
-
 dimethylallyl diphosphate
-
 Aspergillus terreus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.35 45000
-
 6 * 45000, SDS-PAGE Aspergillus terreus
2.5.1.35 240000 270000 gel filtration Aspergillus terreus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.35 additional information Aspergillus terreus enzyme is involved in biosynthesis of aspulvinone pigments ?
-
 ?
2.5.1.35 additional information Aspergillus terreus IAM 2054 enzyme is involved in biosynthesis of aspulvinone pigments ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.35 Aspergillus terreus
-
 IAM 2054
-
2.5.1.35 Aspergillus terreus IAM 2054
-
 IAM 2054
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.35
-
 Aspergillus terreus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.35 mycelium
-
 Aspergillus terreus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.35 0.0294
-
-
 Aspergillus terreus

Storage Stability

EC Number Storage Stability Organism
2.5.1.35 -20°C, stable for 1 week Aspergillus terreus
2.5.1.35 4°C, 50% loss of activity after 3 weeks Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.35 (E)-3-methylpent-2-enyl diphosphate + aspulvinone E 35% of the activity with dimethylallyl diphosphate Aspergillus terreus ?
-
 ?
2.5.1.35 (E)-3-methylpent-2-enyl diphosphate + aspulvinone E 35% of the activity with dimethylallyl diphosphate Aspergillus terreus IAM 2054 ?
-
 ?
2.5.1.35 (E)-butenyl diphosphate + aspulvinone E 10% of the activity with dimethylallyl diphosphate Aspergillus terreus ?
-
 ?
2.5.1.35 cycloheptylideneethyl diphosphate + aspulvinone E 10% of the activity with dimethylallyl diphosphate Aspergillus terreus ?
-
 ?
2.5.1.35 cyclohexylideneethyl diphosphate + aspulvinone E 35% of the activity with dimethylallyl diphosphate Aspergillus terreus ?
-
 ?
2.5.1.35 cyclohexylideneethyl diphosphate + aspulvinone E 35% of the activity with dimethylallyl diphosphate Aspergillus terreus IAM 2054 ?
-
 ?
2.5.1.35 cyclopentylideneethyl diphosphate + aspulvinone E 35% of the activity with dimethylallyl diphosphate Aspergillus terreus ?
-
 ?
2.5.1.35 cyclopentylideneethyl diphosphate + aspulvinone E 35% of the activity with dimethylallyl diphosphate Aspergillus terreus IAM 2054 ?
-
 ?
2.5.1.35 dimethylallyl diphosphate + aspulvinone E
-
 Aspergillus terreus diphosphate + aspulvinone H
-
 ?
2.5.1.35 dimethylallyl diphosphate + aspulvinone E
-
 Aspergillus terreus IAM 2054 diphosphate + aspulvinone H
-
 ?
2.5.1.35 dimethylallyl diphosphate + aspulvinone G
-
 Aspergillus terreus ?
-
 ?
2.5.1.35 dimethylallyl diphosphate + aspulvinone I 75% of the activity with aspulvinone E Aspergillus terreus ?
-
 ?
2.5.1.35 dimethylallyl diphosphate + aspulvinone I-c 13% of the activity with aspulvinone E Aspergillus terreus ?
-
 ?
2.5.1.35 dimethylallyl diphosphate + atromentic acid 7% of the activity with aspulvinone E Aspergillus terreus ?
-
 ?
2.5.1.35 dimethylallyl diphosphate + pulvinone 6% of the activity with aspulvinone E Aspergillus terreus ?
-
 ?
2.5.1.35 additional information enzyme is involved in biosynthesis of aspulvinone pigments Aspergillus terreus ?
-
 ?
2.5.1.35 additional information enzyme is involved in biosynthesis of aspulvinone pigments Aspergillus terreus IAM 2054 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.35 hexamer 6 * 45000, SDS-PAGE Aspergillus terreus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.35 7
-
-
 Aspergillus terreus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.35 0.0178
-
 diphosphate inhibition against dimethylallyl diphosphate Aspergillus terreus
2.5.1.35 0.05
-
 diphosphate inhibition against aspulvinone Aspergillus terreus