EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.3.3 | cDNA encoding the full-length hST6GalNAc-I from HT-29-MTX cells is cloned, stable transfection of MDA-MB-231 breast cancer cells expressing enzyme induces the expression of sialyl-Tn antigen at the cell surface associated with morphological changes, decreased growth and increased migration of the cells | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.3.3 | CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | Homo sapiens | hST6GalNAc-I catalyzes the key step in the biosynthesis of Sialyl-Tn antigen, Neu5Acalpha(2-6)GalNAcalpha1-O-Ser/Thr | CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.3.3 | Homo sapiens | Q9NSC7 | hST6GalNAc-I | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.3.3 | HT-29-MTX cell | colon cancer cells, hST6GalNAc-I | Homo sapiens | - |
2.4.3.3 | additional information | no expression of hST6GalNAc-I in MDA-MB-231, BT-20, MCF-7 and T47-D cells | Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.3.3 | CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | hST6GalNAc-I catalyzes the key step in the biosynthesis of Sialyl-Tn antigen, Neu5Acalpha(2-6)GalNAcalpha1-O-Ser/Thr | Homo sapiens | CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + GTTPSPVPT[GalNAc]TSTTSAP | hexadecaglycopeptide acceptor, corresponding to the MUC5AC tandem repeat sequence substituted by a GalNAc residue on Thr-9, hST6GalNAc-I | Homo sapiens | CMP + GTTPSPVPT[Neu5Acalpha(2-6)GalNAc]TSTTSAP | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | hST6GalNAc-I transfers a sialic acid residue in alpha2,6-linkage to GalNAc-O-Ser/Thr, substrate specificity of hST6GalNAc-I and -II | Homo sapiens | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.3.3 | 37 | - |
assay at | Homo sapiens |