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Literature summary extracted from

  • Higa, H.H.; Paulson, J.C.
    Sialylation of glycoprotein oligosaccharides with N-acetyl-, N-glycolyl-, and N-O-diacetylneuraminic acids (1985), J. Biol. Chem., 260, 8838-8849.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.3 0.08
-
CMP-9-O-acetyl-N-acetylneuraminate 37°C Bos taurus
2.4.3.3 0.1
-
CMP-9-O-acetyl-N-acetylneuraminate 37°C Sus scrofa
2.4.3.3 0.15
-
CMP-N-acetylneuraminate 37°C Bos taurus
2.4.3.3 0.21
-
CMP-N-glycolylneuraminate 37°C Bos taurus
2.4.3.3 0.27
-
CMP-N-acetylneuraminate 37°C Sus scrofa
2.4.3.3 0.52
-
CMP-N-glycolylneuraminate 37°C Sus scrofa
2.4.3.3 4.7
-
submaxillary asialo-mucin ovine, CMP-N-acetylneuraminate as donor, 37°C Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.3.3 120000
-
x * 120000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.3.3 CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein Sus scrofa one of the last steps of the biosynthesis of oligosaccharides of glycoproteins CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
?
2.4.3.3 CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein Bos taurus one of the last steps of the biosynthesis of oligosaccharides of glycoproteins CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Bos taurus
-
-
-
2.4.3.1 Rattus norvegicus
-
-
-
2.4.3.3 Bos taurus
-
-
-
2.4.3.3 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.3
-
Sus scrofa
2.4.3.3 solubilized with Triton X-100, CDP-agarose affinity chromatography, 4500fold Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.1 colostrum
-
Bos taurus
-
2.4.3.3 submaxillary gland
-
Sus scrofa
-
2.4.3.3 submaxillary gland
-
Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.3.3 0.00435
-
37°C Bos taurus
2.4.3.3 28.3
-
37°C Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.4.3.3 -20°C, 10 mM sodium cacodylate, pH 6.5, 50 mM NaCl, 50% glycerol, 0.02% NaN3, at least 6 months, stable Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-9-O-acetyl-N-acetylneuraminate + asialo-alpha1-acid glycoprotein comparable or even higher transfer rates than with CMP-N-acetylneuraminate Rattus norvegicus ?
-
?
2.4.3.1 CMP-9-O-acetyl-N-acetylneuraminate + asialo-alpha1-acid glycoprotein comparable or even higher transfer rates than with CMP-N-acetylneuraminate Bos taurus ?
-
?
2.4.3.1 CMP-N-acetylneuraminate + asialo-orosomucoid CMP-N-acetylneuraminate can be replaced with comparable or even higher transfer rates by CMP-N-glycolylneuraminate, CMP-9-O-acetyl-N-acetylneuraminate Rattus norvegicus CMP + orosomucoid
-
?
2.4.3.1 CMP-N-acetylneuraminate + asialo-orosomucoid CMP-N-acetylneuraminate can be replaced with comparable or even higher transfer rates by CMP-N-glycolylneuraminate, CMP-9-O-acetyl-N-acetylneuraminate Bos taurus CMP + orosomucoid
-
?
2.4.3.1 CMP-N-acetylneuraminate + asialo-orosomucoid i.e. asialo-alpha1 acid glycoprotein Rattus norvegicus CMP + orosomucoid
-
?
2.4.3.1 CMP-N-acetylneuraminate + asialo-orosomucoid i.e. asialo-alpha1 acid glycoprotein Bos taurus CMP + orosomucoid
-
?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R transfers sialic acid to terminal positions on N-linked glycans Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R transfers sialic acid to terminal positions on N-linked glycans Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
?
2.4.3.1 CMP-N-glycolylneuraminate + asialo-alpha1-acid glycoprotein comparable or even higher transfer rates than with CMP-N-acetylneuraminate Rattus norvegicus ?
-
?
2.4.3.1 CMP-N-glycolylneuraminate + asialo-alpha1-acid glycoprotein comparable or even higher transfer rates than with CMP-N-acetylneuraminate Bos taurus ?
-
?
2.4.3.3 CMP-9-O-acetyl-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein poor donor, 10% as effective as CMP-N-acetylneuraminate Sus scrofa CMP + R-(2,6-9-O-acetyl-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
?
2.4.3.3 CMP-9-O-acetyl-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein poor donor, 10% as effective as CMP-N-acetylneuraminate Bos taurus CMP + R-(2,6-9-O-acetyl-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
?
2.4.3.3 CMP-N-acetylneuraminate + antifreeze glycoprotein
-
Sus scrofa ?
-
?
2.4.3.3 CMP-N-acetylneuraminate + antifreeze glycoprotein
-
Bos taurus ?
-
?
2.4.3.3 CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein one of the last steps of the biosynthesis of oligosaccharides of glycoproteins Sus scrofa CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
?
2.4.3.3 CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein one of the last steps of the biosynthesis of oligosaccharides of glycoproteins Bos taurus CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
?
2.4.3.3 CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein CMP-N-glycolylneuraminate has the same efficiency as CMP-N-acetylneuraminate, CMP-9-O-acetyl-N-acetylneuraminate has much lower efficiency than CMP-N-acetylneuraminate Sus scrofa CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
?
2.4.3.3 CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein CMP-N-glycolylneuraminate has the same efficiency as CMP-N-acetylneuraminate, CMP-9-O-acetyl-N-acetylneuraminate has much lower efficiency than CMP-N-acetylneuraminate Bos taurus CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
?
2.4.3.3 CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein CMP-N-glycolylneuraminate can replace CMP-N-acetylneuraminate Sus scrofa CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
?
2.4.3.3 CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein CMP-N-glycolylneuraminate can replace CMP-N-acetylneuraminate Bos taurus CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
?
2.4.3.3 CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein antifreeze glycoprotein Sus scrofa CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
?
2.4.3.3 CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein antifreeze glycoprotein Bos taurus CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
?
2.4.3.3 CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein ovine submaxillary asialo-mucin, with simple O-linked GalNAcalpha-Thr/Ser Sus scrofa CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
?
2.4.3.3 CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein ovine submaxillary asialo-mucin, with simple O-linked GalNAcalpha-Thr/Ser Bos taurus CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
?
2.4.3.3 CMP-N-acetylneuraminate + submaxillary asialo-mucin ovine submaxillary asialo-mucin, with simple O-linked GalNAcalpha-Thr/Ser Sus scrofa CMP + submaxillary mucin linkage formed is NeuAc(2-6)GalNAcalpha(1-)O-Thr/Ser ?
2.4.3.3 CMP-N-acetylneuraminate + submaxillary asialo-mucin ovine submaxillary asialo-mucin, with simple O-linked GalNAcalpha-Thr/Ser Bos taurus CMP + submaxillary mucin linkage formed is NeuAc(2-6)GalNAcalpha(1-)O-Thr/Ser ?
2.4.3.3 CMP-N-glycolylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein CMP-N-glycolylneuraminate can replace CMP-N-acetylneuraminate with the same efficiency Sus scrofa CMP + R-(2,6-alpha-N-glycolylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
?
2.4.3.3 CMP-N-glycolylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein CMP-N-glycolylneuraminate can replace CMP-N-acetylneuraminate with the same efficiency Bos taurus CMP + R-(2,6-alpha-N-glycolylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
?

Subunits

EC Number Subunits Comment Organism
2.4.3.3 ?
-
Sus scrofa
2.4.3.3 ? x * 120000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.3.1 37
-
assay at Rattus norvegicus
2.4.3.1 37
-
assay at Bos taurus
2.4.3.3 37
-
assay at Sus scrofa
2.4.3.3 37
-
assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.3.1 6.5
-
assay at Rattus norvegicus
2.4.3.1 6.5
-
assay at Bos taurus