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Literature summary extracted from

  • Yonaha, K.; Misono, H.; Yamamoto, T.; Soda, K.
    D-amino acid aminotransferase of Bacillus sphaericus. Enzymologic and spectrometric properties (1975), J. Biol. Chem., 250, 6983-6989.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.21
-
 Lysinibacillus sphaericus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.21 3-Methyl-2-benzothiazolone hydrazone hydrochloride
-
 Lysinibacillus sphaericus
2.6.1.21 Aminoxyacetate 0.01 mM, 90% inhibition Lysinibacillus sphaericus
2.6.1.21 beta-Chloro-D-alanine 0.025, 56% inhibition Lysinibacillus sphaericus
2.6.1.21 D-cycloserine
-
 Lysinibacillus sphaericus
2.6.1.21 D-penicillamine 1 mM, 73% inhibition Lysinibacillus sphaericus
2.6.1.21 HgCl2 0.1 mM, complete inhibition Lysinibacillus sphaericus
2.6.1.21 hydroxylamine 0.01 mM, 61% inhibition Lysinibacillus sphaericus
2.6.1.21 L-Penicillamine 1 mM, 28% inhibition Lysinibacillus sphaericus
2.6.1.21 N-ethylmaleimide 1 mM, 50% inhibition Lysinibacillus sphaericus
2.6.1.21 phenylhydrazine 2 mM, 85% inhibition Lysinibacillus sphaericus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.21 1.3
-
 D-alanine pH 8.0, 37°C, cosubstrate 2-oxobutanoate Lysinibacillus sphaericus
2.6.1.21 3.4
-
 2-oxoglutarate pH 8.0, 37°C, cosubstrate D-alanine Lysinibacillus sphaericus
2.6.1.21 4.2
-
 D-alanine pH 8.0, 37°C, cosubstrate 2-oxoglutarate Lysinibacillus sphaericus
2.6.1.21 14
-
 2-oxobutanoate cosubstrate D-alanine Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.21 58000
-
 sedimentation equilibrium centrifugation Lysinibacillus sphaericus
2.6.1.21 60000
-
 gel filtration Lysinibacillus sphaericus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.21 Lysinibacillus sphaericus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.21 D-2-aminobutanoate + pyruvate
-
 Lysinibacillus sphaericus 2-oxobutanoate + D-alanine
-
 ?
2.6.1.21 D-alanine + 2-oxoglutarate
-
 Lysinibacillus sphaericus pyruvate + D-glutamate
-
 r
2.6.1.21 D-aminobutanoate + 2-oxoglutarate 97% of the activity with D-alanine Lysinibacillus sphaericus oxobutanoate + D-glutamate
-
 ?
2.6.1.21 D-arginine + 2-oxoglutarate 6% of the activity with D-alanine Lysinibacillus sphaericus ? + D-glutamate
-
 ?
2.6.1.21 D-asparagine + 2-oxoglutarate 31% of the activity with D-alanine Lysinibacillus sphaericus 2-oxosuccinamate + D-glutamate
-
 ?
2.6.1.21 D-asparagine + pyruvate
-
 Lysinibacillus sphaericus 2-oxosuccinamate + D-alanine
-
 ?
2.6.1.21 D-aspartate + 2-oxoglutarate 30% of the activity with D-alanine Lysinibacillus sphaericus 2-oxosuccinate + D-glutamate
-
 ?
2.6.1.21 D-aspartate + pyruvate
-
 Lysinibacillus sphaericus 2-oxosuccinate + D-alanine
-
 ?
2.6.1.21 D-ethionine + 2-oxoglutarate 76% of the activity with D-alanine Lysinibacillus sphaericus 4-ethylsulfanyl-2-oxobutanoate + D-glutamate
-
 ?
2.6.1.21 D-glutamate + 2-oxobutanoate
-
 Lysinibacillus sphaericus 2-oxoglutarate + 2-aminobutanoate
-
 ?
2.6.1.21 D-glutamate + pyruvate
-
 Lysinibacillus sphaericus 2-oxoglutarate + D-alanine
-
 r
2.6.1.21 D-glutamine + 2-oxoglutarate 52% of the activity with D-alanine Lysinibacillus sphaericus 4-carbamoyl-2-oxobutanoate + D-glutamate
-
 ?
2.6.1.21 D-histidine + 2-oxoglutarate 6% of the activity with D-alanine Lysinibacillus sphaericus 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate
-
 ?
2.6.1.21 D-leucine + 2-oxoglutarate 8% of the activity with D-alanine Lysinibacillus sphaericus 4-methyl-2-oxopentanoate + D-glutamate
-
 ?
2.6.1.21 D-lysine + pyruvate weak activity Lysinibacillus sphaericus ? + D-alanine
-
 ?
2.6.1.21 D-methionine + 2-oxoglutarate 61% of the activity with D-alanine Lysinibacillus sphaericus 4-methylsulfanyl-2-oxobutanoate + D-glutamate
-
 ?
2.6.1.21 D-methionine + pyruvate
-
 Lysinibacillus sphaericus 4-methylsulfanyl-2-oxobutanoate + L-alanine
-
 ?
2.6.1.21 D-norleucine + 2-oxoglutarate 46% of the activity with D-alanine Lysinibacillus sphaericus 2-oxopentanoate + D-glutamate
-
 ?
2.6.1.21 D-norvaline + 2-oxoglutarate 83% of the activity with D-alanine Lysinibacillus sphaericus 2-oxopentanoate + D-glutamate
-
 ?
2.6.1.21 D-norvaline + pyruvate
-
 Lysinibacillus sphaericus 2-oxopentanoate + D-alanine
-
 ?
2.6.1.21 D-ornithine + 2-oxoglutarate 16% of activity with D-alanine Lysinibacillus sphaericus ? + D-glutamate
-
 ?
2.6.1.21 D-ornithine + pyruvate
-
 Lysinibacillus sphaericus ? + L-alanine
-
 ?
2.6.1.21 D-phenylalanine + 2-oxoglutarate 32% of the activity with D-alanine Lysinibacillus sphaericus phenylpyruvate + D-glutamate
-
 ?
2.6.1.21 D-phenylalanine + pyruvate
-
 Lysinibacillus sphaericus phenylpyruvate + D-alanine
-
 ?
2.6.1.21 D-theanine + 2-oxoglutarate 40% of the activity with D-alanine Lysinibacillus sphaericus ? + D-glutamate
-
 ?
2.6.1.21 D-tryptophan + 2-oxoglutarate 4% of the activity with D-alanine Lysinibacillus sphaericus 3-indole-2-oxopropanoate + D-glutamate
-
 ?
2.6.1.21 D-tryptophan + pyruvate weak activity Lysinibacillus sphaericus 3-indole-2-oxopropanoate + D-alanine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.21 dimer
-
 Lysinibacillus sphaericus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.21 37
-
 assay at Lysinibacillus sphaericus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.21 pyridoxal 5'-phosphate a pyridoxal phosphate protein Lysinibacillus sphaericus
2.6.1.21 pyridoxal 5'-phosphate 2 mol of pyridoxal 5'-phosphate per mol of enzyme Lysinibacillus sphaericus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.21 0.000011
-
 D-cycloserine pH 8.0, 37°C Lysinibacillus sphaericus
2.6.1.21 0.00225
-
 beta-Chloro-D-alanine pH 8.0, 37°C Lysinibacillus sphaericus
2.6.1.21 0.057
-
 D-penicillamine pH 8.0, 37°C Lysinibacillus sphaericus
2.6.1.21 0.8
-
 L-Penicillamine pH 8.0, 37°C Lysinibacillus sphaericus