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Literature summary extracted from

  • Eze, L.C.; Echetebu, C.O.
    Some properties of aspartate and alanine aminotransferases from Trichoderma virie (1980), J. Gen. Microbiol., 120, 523-527.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.1 Hg2+
-
 Trichoderma viride
2.6.1.1 succinate
-
 Trichoderma viride
2.6.1.2 Hg2+ 93% inhibition Trichoderma viride
2.6.1.2 succinate competitive inhibitor Trichoderma viride

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.2 1.85
-
 2-oxoglutarate pH 8.6, 37°C Trichoderma viride
2.6.1.2 8.3
-
 DL-alanine pH 8.6, 37°C Trichoderma viride

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate Trichoderma viride
-
 oxaloacetate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.1 Trichoderma viride
-
-
-
2.6.1.2 Trichoderma viride
-
 soil fungus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.1 partial Trichoderma viride
2.6.1.2 partial Trichoderma viride

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.1 mycelium
-
 Trichoderma viride
-
2.6.1.2 mycelium
-
 Trichoderma viride
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.1 glycine + 2-oxoglutarate 7.7% of the activity with aspartate Trichoderma viride glyoxylate + L-glutamate
-
 ?
2.6.1.1 L-alanine + 2-oxoglutarate 7.7% of the activity with aspartate Trichoderma viride pyruvate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Trichoderma viride oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate best amino acid donor Trichoderma viride oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-cysteine + 2-oxoglutarate 26.2% of the activity with L-aspartate Trichoderma viride 3-mercaptopyruvate + L-glutamate
-
 ?
2.6.1.1 L-methionine + 2-oxoglutarate 93% of the activity with aspartate Trichoderma viride L-glutamate + 4-methylsulfanyl-2-oxobutyric acid
-
 ?
2.6.1.1 L-phenylalanine + 2-oxoglutarate 7.7% of the activity with L-aspartate Trichoderma viride 2-oxo-3-phenylpropionic acid + L-glutamate i.e. phenylpyruvate ?
2.6.1.1 L-serine + 2-oxoglutarate 7.7% of the activity with aspartate Trichoderma viride 3-hydroxy-2-oxopropionic acid + L-glutamate
-
 ?
2.6.1.1 L-tyrosine + 2-oxoglutarate 84.6% of the activity with aspartate Trichoderma viride 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate i.e. 4-hydroxyphenylpyruvate ?
2.6.1.2 DL-alanine + 2-oxoglutarate
-
 Trichoderma viride pyruvate + L-glutamate
-
 ?
2.6.1.2 glycine + 2-oxoglutarate 24% of activity with DL-alanine Trichoderma viride glyoxylate + glutamate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Trichoderma viride pyruvate + L-glutamate
-
 ?
2.6.1.2 L-methionine + 2-oxoglutarate 75% of activity with DL-alanine Trichoderma viride 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
 ?
2.6.1.2 serine + 2-oxoglutarate 22% of activity with DL-alanine Trichoderma viride 3-hydroxy-2-oxopropanoate + glutamate
-
 ?
2.6.1.2 tyrosine + 2-oxoglutarate 36.5% of activity with DL-alanine Trichoderma viride 3-(4-hydroxyphenyl)-2-oxopropanoate + glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.1 GOT
-
 Trichoderma viride

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.1 37
-
 relatively stable Trichoderma viride
2.6.1.1 50
-
 30 min, 20% loss of activity Trichoderma viride
2.6.1.1 60
-
 30 min, 75% loss of activity Trichoderma viride
2.6.1.1 70
-
 30 min, 80% loss of activity Trichoderma viride

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.1 9
-
-
 Trichoderma viride
2.6.1.2 8.6
-
-
 Trichoderma viride

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.2 17
-
 succinate pH 8.6, 37°C Trichoderma viride