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Literature summary extracted from

  • Noguchi, T.; Takada, Y.; Kido, R.
    Glutamate-glyoxylate aminotransferase in rat liver cytosol. Purification, properties and identity with alanine-2-oxoglutarate aminotransferase (1977), Hoppe-Seyler's Z. Physiol. Chem., 358, 1533-1542.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.2 Hg2+
-
 Rattus norvegicus
2.6.1.2 hydroxylamine 1 mM, 96% inhibition Rattus norvegicus
2.6.1.2 Isonicotinic hydrazide
-
 Rattus norvegicus
2.6.1.2 KCN
-
 Rattus norvegicus
2.6.1.2 Semicarbazide 1 mM, 40% inhibition Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.2 112000
-
 sucrose density gradient centrifugation Rattus norvegicus
2.6.1.2 112000 118000 gel filtration Homo sapiens
2.6.1.2 112000 118000 gel filtration Canis lupus familiaris
2.6.1.2 112000 118000 gel filtration Felis catus
2.6.1.2 115000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.2 Canis lupus familiaris
-
-
-
2.6.1.2 Felis catus
-
-
-
2.6.1.2 Homo sapiens
-
-
-
2.6.1.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.2 heat treatment, ammonium sulfate, DEAE-cellulose, isoelectric focusing, Sephadex G-150, hydroxylapatite, DEAE-cellulose Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.2 liver
-
 Homo sapiens
-
2.6.1.2 liver
-
 Rattus norvegicus
-
2.6.1.2 liver
-
 Canis lupus familiaris
-
2.6.1.2 liver
-
 Felis catus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.2 480
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.6.1.2 -20°C, 50 mM potassium phosphate buffer, pH 7.5, 5 mM 2-mercaptoethanol, 5% glycerol, stable for at least 5 weeks Rattus norvegicus
2.6.1.2 0-5°C, 50 mM potassium phosphate buffer, pH 7.5, 5 mM 2-mercaptoethanol, 5% glycerol, stable for 2 weeks Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.2 glyoxylate + alanine 70% of activity with glutamate Rattus norvegicus glycine + pyruvate
-
 ir
2.6.1.2 glyoxylate + glutamate
-
 Rattus norvegicus glycine + 2-oxoglutarate
-
 ir
2.6.1.2 glyoxylate + glutamine 42% of activity with glutamate Rattus norvegicus glycine + 2-oxosuccinamate
-
 ?
2.6.1.2 glyoxylate + methionine 37% of activity with glutamate Rattus norvegicus glycine + 4-methylsulfanyl-2-oxobutanoate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Homo sapiens pyruvate + L-glutamate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Canis lupus familiaris pyruvate + L-glutamate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Felis catus pyruvate + L-glutamate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate with 2-oxoglutarate specific for L-alanine Rattus norvegicus pyruvate + L-glutamate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate glutamate-glyoxylate aminotransferase is identical with alanine-2-oxoglutarate aminotransferase Rattus norvegicus pyruvate + L-glutamate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.2 37
-
 assay at Homo sapiens
2.6.1.2 37
-
 assay at Rattus norvegicus
2.6.1.2 37
-
 assay at Canis lupus familiaris
2.6.1.2 37
-
 assay at Felis catus