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Literature summary extracted from

  • Ireland, R.J.; Joy, K.W.
    Purification and properties of an asparagine aminotransferase from Pisum sativum leaves (1983), Arch. Biochem. Biophys., 223, 291-296.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.45 Aminooxyacetate 1 mM, complete inhibition Pisum sativum
2.6.1.45 glyoxylate competitive vs. pyruvate Pisum sativum
2.6.1.45 hydroxylamine 0.1 mM, 97% inhibition, 85% inhibition in the presence of 0.1 mM pyridoxal phosphate Pisum sativum
2.6.1.45 L-asparagine competitive vs. serine Pisum sativum
2.6.1.45 L-serine competitive vs. asparagine Pisum sativum
2.6.1.45 pyruvate competitive vs. glyoxylate Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.45 0.5
-
L-serine pH 8.1, 30°C, cosubstrate pyruvate Pisum sativum
2.6.1.45 0.6
-
L-serine pH 8.1, 30°C, cosubstrate glyoxylate Pisum sativum
2.6.1.45 2
-
pyruvate pH 8.1, 30°C, cosubstrate L-serine Pisum sativum
2.6.1.45 2.5
-
pyruvate pH 8.1, 30°C, cosubstrates: L-asparagine, glyoxylate, L-asparagine Pisum sativum
2.6.1.45 3.9
-
L-asparagine pH 8.1, 30°C, cosubstrate glyoxylate Pisum sativum
2.6.1.45 4.5
-
L-asparagine pH 8.1, 30°C, cosubstrate pyruvate Pisum sativum
2.6.1.45 4.6
-
glyoxylate pH 8.1, 30°C, cosubstrate L-serine Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.45 peroxisome
-
Pisum sativum 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.45 105000
-
gel filtration Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.45 L-serine + glyoxylate Pisum sativum
-
3-hydroxypyruvate + glycine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.45 Pisum sativum
-
pea, cv. Little Marvel
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.45 pH 5.4, polyethylene glycol 6000, DEAE-Sephadex A25, Sephacryl S-300, partial purification Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.45 leaf
-
Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.45 39.3
-
-
Pisum sativum

Storage Stability

EC Number Storage Stability Organism
2.6.1.45 5°C, stable overnight, 20% loss of activity per day on further storage, in 20% ethylene glycol or 15% glycerol, 20% loss of activity within 5 days Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.45 L-asparagine + glyoxylate transamination at 38% the rate of L-serine Pisum sativum 2-oxosuccinamate + glycine
-
r
2.6.1.45 L-serine + 2-oxosuccinamate poor substrate Pisum sativum 3-hydroxypyruvate + L-asparagine
-
?
2.6.1.45 L-serine + glyoxylate
-
Pisum sativum 3-hydroxypyruvate + glycine
-
?
2.6.1.45 L-serine + glyoxylate no activity with Phe, Arg, Val, Trp, Thr, Met Pisum sativum 3-hydroxypyruvate + glycine
-
r
2.6.1.45 L-serine + glyoxylate reverse reaction at 4-11% the rate of forward reaction Pisum sativum 3-hydroxypyruvate + glycine
-
r
2.6.1.45 L-serine + glyoxylate preferred substrates Pisum sativum 3-hydroxypyruvate + glycine
-
r
2.6.1.45 L-serine + glyoxylate no or trace activity with oxaloacetate, 2-oxoglutarate Pisum sativum 3-hydroxypyruvate + glycine
-
r
2.6.1.45 L-serine + glyoxylate no activity with Tyr, Ile, Pro, Cys, Leu, Asp Pisum sativum 3-hydroxypyruvate + glycine
-
r
2.6.1.45 L-serine + glyoxylate equilibrium towards glycine production Pisum sativum 3-hydroxypyruvate + glycine
-
r
2.6.1.45 L-serine + hydroxypyruvate poor substrate Pisum sativum 3-hydroxypyruvate + L-serine
-
?
2.6.1.45 L-serine + pyruvate 8% of glyoxylate transamination activity Pisum sativum 3-hydroxypyruvate + L-alanine
-
r
2.6.1.45 L-serine + pyruvate equilibrium towards alanine production Pisum sativum 3-hydroxypyruvate + L-alanine
-
r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.45 30
-
assay at Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.45 8.1
-
-
Pisum sativum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.45 7.5 9.2 approx. 50% activity at pH 7.5 and 9.2, no activity below pH 6.7 and above pH 9.9 Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.45 pyridoxal 5'-phosphate required for activity Pisum sativum
2.6.1.45 pyridoxal 5'-phosphate Km-value: 0.0025 mM Pisum sativum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.45 0.12
-
L-serine pH 8.1, 30°C Pisum sativum
2.6.1.45 0.4
-
glyoxylate pH 8.1, 30°C Pisum sativum
2.6.1.45 3
-
L-asparagine pH 8.1, 30°C Pisum sativum
2.6.1.45 25
-
pyruvate pH 8.1, 30°C Pisum sativum