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Literature summary extracted from

  • Smith, I.K.
    Purification and characterization of serine:glyoxylate aminotransferase from kidney bean (Phaseolus vulgaris) (1973), Biochim. Biophys. Acta, 321, 156-164.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.45 (NH4)2SO4 0.05 mM, 45% inhibition, 5 mM, complete inhibition Phaseolus vulgaris
2.6.1.45 ammonium acetate 0.1 mM, 42% inhibition, 10 mM, complete inhibition Phaseolus vulgaris
2.6.1.45 hydroxylamine 0.01 mM, 98% inhibition of serine-glyoxylate transamination Phaseolus vulgaris
2.6.1.45 N-ethylmaleimide 1 mM, 41% inhibition Phaseolus vulgaris
2.6.1.45 NH4Cl 0.1 mM, 42% inhibition, 10 mM, complete inhibition Phaseolus vulgaris
2.6.1.45 p-chloromercuribenzoate 1 mM, 37% inhibition Phaseolus vulgaris
2.6.1.51 hydroxylamine
-
 Phaseolus vulgaris
2.6.1.51 additional information less sensitive to N-ethylmaleimide and p-chloromercuribenzoate, not inhibited by NH4+ Phaseolus vulgaris
2.6.1.51 N-ethylmaleimide
-
 Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.45 additional information
-
 additional information kinetic study Phaseolus vulgaris
2.6.1.45 0.39
-
 L-serine cosubstrate pyruvate Phaseolus vulgaris
2.6.1.45 0.6
-
 glyoxylate pH 8.0, 30°C, cosubstrate L-serine Phaseolus vulgaris
2.6.1.45 0.71
-
 L-serine pH 8.0, 30°C, cosubstrate glyoxylate Phaseolus vulgaris
2.6.1.51 0.39
-
 serine pH 8.0, 30°C Phaseolus vulgaris
2.6.1.51 38
-
 pyruvate pH 8.0, 30°C Phaseolus vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.45 soluble
-
 Phaseolus vulgaris
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.45 L-serine + glyoxylate Phaseolus vulgaris involved in glycine metabolism 3-hydroxypyruvate + glycine
-
 ?
2.6.1.51 L-serine + pyruvate Phaseolus vulgaris
-
 3-hydroxypyruvate + L-alanine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.45 Phaseolus vulgaris
-
 kidney bean, cv. Red Kidney
-
2.6.1.51 Phaseolus vulgaris
-
 kidney bean, L. cv. Red Kidney
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.45 DEAE-cellulose, agarose gel, Brushite, Sephadex g-200, partial purification Phaseolus vulgaris
2.6.1.51 co-purification of serine:glyoxylate aminotransferase Phaseolus vulgaris

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.45 L-serine + glyoxylate = 3-hydroxypyruvate + glycine ping-pong reaction mechanism Phaseolus vulgaris
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.45 leaf
-
 Phaseolus vulgaris
-
2.6.1.51 leaf
-
 Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.45 6.97
-
-
 Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.45 L-serine + glyoxylate preferred substrates Phaseolus vulgaris 3-hydroxypyruvate + glycine
-
 r
2.6.1.45 L-serine + glyoxylate involved in glycine metabolism Phaseolus vulgaris 3-hydroxypyruvate + glycine
-
 ?
2.6.1.45 L-serine + pyruvate
-
 Phaseolus vulgaris 3-hydroxypyruvate + L-alanine
-
 ?
2.6.1.51 glycine + 3-hydroxypyruvate
-
 Phaseolus vulgaris glyoxylate + L-serine
-
 r
2.6.1.51 L-serine + glyoxylate
-
 Phaseolus vulgaris glycine + 3-hydroxypyruvate
-
 r
2.6.1.51 L-serine + pyruvate
-
 Phaseolus vulgaris 3-hydroxypyruvate + L-alanine
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.45 30
-
 assay at Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.45 8.2
-
-
 Phaseolus vulgaris