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Literature summary extracted from

  • Toyama, S.; Misono, H.; Soda, K.
    Properties of taurine: alpha-ketoglutarate aminotransferase of Achromobacter superficialis. Inactivation and reactivation of enzyme (1978), Biochim. Biophys. Acta, 523, 75-81.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.55 3-Methyl-2-benzothiazolone hydrazone hydrochloride weak inhibition Achromobacter superficialis
2.6.1.55 D-cycloserine 0.5 mM; 20% inhibition Achromobacter superficialis
2.6.1.55 HgCl2 0.1 mM, 98% inhibition Achromobacter superficialis
2.6.1.55 hydroxylamine 0.5 mM, complete inhibition Achromobacter superficialis
2.6.1.55 L-Cycloserine 0.5 mM, 97% inhibition Achromobacter superficialis
2.6.1.55 additional information not inhibited by p-chloromercuribenzoate, monoiodoacetic acid, D-penicillamine, L-penicillamine and EDTA Achromobacter superficialis
2.6.1.55 phenylhydrazine 0.5 mM, 17% inhibition Achromobacter superficialis
2.6.1.55 Semicarbazide 0.5 mM, 21% inhibition Achromobacter superficialis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.55 11
-
2-oxoglutarate pH 8.0, 37°C, cosubstrate DL-3-aminoisobutyrate Achromobacter superficialis
2.6.1.55 12
-
taurine
-
Achromobacter superficialis
2.6.1.55 16
-
Hypotaurine
-
Achromobacter superficialis
2.6.1.55 17
-
beta-Alanine
-
Achromobacter superficialis

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.55 Achromobacter superficialis
-
-
-

Storage Stability

EC Number Storage Stability Organism
2.6.1.55 0-5°C, crystalline enzyme in 10 mM potassium phosphate buffer, pH 7.0, 60% saturation of ammonium sulfate, 100 mM pyridoxal 5'-phosphate, 0.01% 2-mercaptoethanol, several months, no loss of activity Achromobacter superficialis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.55 3-aminopropanesulfonate + 2-oxoglutarate 43% of the activity with taurine Achromobacter superficialis 2-oxopropanesulfonate + L-glutamate
-
?
2.6.1.55 4-aminobutyrate + 2-oxoglutarate 60% of the activity with taurine Achromobacter superficialis L-glutamate + acetaldehyde + sulfite
-
?
2.6.1.55 beta-alanine + 2-oxoglutarate
-
Achromobacter superficialis 3-oxopropanoate + L-glutamate
-
?
2.6.1.55 DL-3-aminobutyrate + 2-oxoglutarate 14% of the activity with taurine Achromobacter superficialis 3-oxobutyrate + L-glutamate
-
?
2.6.1.55 DL-3-aminoisobutyrate + 2-oxoglutarate
-
Achromobacter superficialis 2-methyl-3-oxopropanoate + L-glutamate
-
?
2.6.1.55 hypotaurine + 2-oxoglutarate 601% of the activity with taurine Achromobacter superficialis 2-oxoethanesulfinic acid + L-glutamate
-
?
2.6.1.55 taurine + 2-oxoglutarate no activity with pyruvate, phenylpyruvate, oxaloacetate Achromobacter superficialis sulfoacetaldehyde + L-glutamate
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.55 60
-
pH 6.0-9.0, 10 min, stable Achromobacter superficialis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.55 7.8 8
-
Achromobacter superficialis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.55 6 9 60°C, 10 min, stable Achromobacter superficialis

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.55 pyridoxal 5'-phosphate Km: 0.005 mM Achromobacter superficialis
2.6.1.55 pyridoxal 5'-phosphate 4 mol of pyridoxal phosphate per mol of enzyme Achromobacter superficialis
2.6.1.55 pyridoxal 5'-phosphate enzyme inactivated by treatment with ammonium sulfate is activated by incubation with pyridoxal 5'-phosphate at 45-60°C for about 10 min Achromobacter superficialis