EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.57 | expression in Escherichia coli | Pyrococcus horikoshii |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.6.1.57 | hanging drop vapor diffusion, an equi-volume of 3 M 1,6-hexanediol solution at pH 7.5, 100 mM HEPES buffer, containing 10 mM MgCl2 is added to a protein solution containing 1.6% AeATPh and 0.02 mM pyridoxal 5'-phosphate, a droplet of the solution is equilibrated with 1 ml of 3 M 1,6-hexane-di-ol solution, crystals are grown at room temperature for 1 week, crystal structure of the native enzyme 2.1 a resolution, heavy atom derivatives diffract to 3.0 A resolution | Pyrococcus horikoshii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.57 | 42000 | - |
2 * 42000, recombinant enzyme, SDS-PAGE | Pyrococcus horikoshii |
2.6.1.57 | 56000 | - |
recombinant enzyme, gel filtration | Pyrococcus horikoshii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.57 | Pyrococcus horikoshii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.57 | recombinant enzyme, 80°C, HiTrap Q, HiLoad superdex 200 | Pyrococcus horikoshii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.57 | L-phenylalanine + 2-oxoglutarate | - |
Pyrococcus horikoshii | phenylpyruvate + L-glutamate | - |
? | |
2.6.1.57 | L-tryptophan + 2-oxoglutarate | - |
Pyrococcus horikoshii | 3-indole-2-oxopropanoate + L-glutamate | - |
? | |
2.6.1.57 | L-tyrosine + 2-oxoglutarate | - |
Pyrococcus horikoshii | p-hydroxyphenylpyruvate + L-glutamate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.6.1.57 | dimer | 2 * 42000, recombinant enzyme, SDS-PAGE | Pyrococcus horikoshii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.57 | ArATPh | - |
Pyrococcus horikoshii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.57 | 90 | - |
- |
Pyrococcus horikoshii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.57 | 95 | - |
no loss of activity after 30 min at pH 6.5 | Pyrococcus horikoshii |
2.6.1.57 | 110 | - |
half-life at pH 6.5: 30 min | Pyrococcus horikoshii |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.57 | 4 | 11 | no loss of activity after 24 h at 25°C | Pyrococcus horikoshii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.57 | pyridoxal 5'-phosphate | - |
Pyrococcus horikoshii |