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Literature summary extracted from

  • Matsui, I.; Matsui, E.; Sakai, Y.; Kikuchi, H.; Kawarabayasi, Y.; Ura, H.; Kawaguchi, S.I.; Kuramitsu, S.; Harata, K.
    The molecular structure of hyperthermostable aromatic aminotransferase with novel substrate specificity from Pyrococcus horikoshii (2000), J. Biol. Chem., 275, 4871-4879.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.57 expression in Escherichia coli Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.57 hanging drop vapor diffusion, an equi-volume of 3 M 1,6-hexanediol solution at pH 7.5, 100 mM HEPES buffer, containing 10 mM MgCl2 is added to a protein solution containing 1.6% AeATPh and 0.02 mM pyridoxal 5'-phosphate, a droplet of the solution is equilibrated with 1 ml of 3 M 1,6-hexane-di-ol solution, crystals are grown at room temperature for 1 week, crystal structure of the native enzyme 2.1 a resolution, heavy atom derivatives diffract to 3.0 A resolution Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.57 42000
-
2 * 42000, recombinant enzyme, SDS-PAGE Pyrococcus horikoshii
2.6.1.57 56000
-
recombinant enzyme, gel filtration Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.57 Pyrococcus horikoshii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.57 recombinant enzyme, 80°C, HiTrap Q, HiLoad superdex 200 Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.57 L-phenylalanine + 2-oxoglutarate
-
Pyrococcus horikoshii phenylpyruvate + L-glutamate
-
?
2.6.1.57 L-tryptophan + 2-oxoglutarate
-
Pyrococcus horikoshii 3-indole-2-oxopropanoate + L-glutamate
-
?
2.6.1.57 L-tyrosine + 2-oxoglutarate
-
Pyrococcus horikoshii p-hydroxyphenylpyruvate + L-glutamate
-
?

Subunits

EC Number Subunits Comment Organism
2.6.1.57 dimer 2 * 42000, recombinant enzyme, SDS-PAGE Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
2.6.1.57 ArATPh
-
Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.57 90
-
-
Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.57 95
-
no loss of activity after 30 min at pH 6.5 Pyrococcus horikoshii
2.6.1.57 110
-
half-life at pH 6.5: 30 min Pyrococcus horikoshii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.57 4 11 no loss of activity after 24 h at 25°C Pyrococcus horikoshii

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.57 pyridoxal 5'-phosphate
-
Pyrococcus horikoshii