Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Weigent, D.A.; Nester, E.W.
    Purification and properties of two aromatic aminotransferases in Bacillus subtilis (1976), J. Biol. Chem., 251, 6974-6980.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.57 2-oxoglutarate or pyridoxal 5'-phosphate stabilizes against heat inactivation Bacillus subtilis
2.6.1.57 stable to freezing and thawing Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.9 L-glutamate competitive to tyrosine Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.9 0.15
-
 L-histidinol phosphate
-
 Bacillus subtilis
2.6.1.9 0.71
-
 L-tyrosine
-
 Bacillus subtilis
2.6.1.9 2.5
-
 L-phenylalanine
-
 Bacillus subtilis
2.6.1.9 7.1
-
 2-oxoglutarate
-
 Bacillus subtilis
2.6.1.57 0.025
-
 pyridoxal 5'-phosphate pH 7.5, 37°C Bacillus subtilis
2.6.1.57 2.7
-
 L-tyrosine pH 7.5, 37°C Bacillus subtilis
2.6.1.57 9.8
-
 L-phenylalanine pH 7.5, 37°C Bacillus subtilis
2.6.1.57 38
-
 2-oxoglutarate pH 7.5, 37°C Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.9 33000
-
 gel filtration Bacillus subtilis
2.6.1.57 63500
-
 gel filtration Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.9 Bacillus subtilis
-
-
-
2.6.1.57 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.9 220fold Bacillus subtilis
2.6.1.57 55°C, ammonium sulfate, DE52, phenylalanine-Sepharose, Sephadex G-150 Bacillus subtilis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.9 L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate ping-pong bi bi mechanism Bacillus subtilis
a
2.6.1.57 an aromatic amino acid + 2-oxoglutarate = an aromatic oxo acid + L-glutamate ping pong mechanism Bacillus subtilis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.9 325
-
-
 Bacillus subtilis
2.6.1.57 95
-
-
 Bacillus subtilis
2.6.1.57 950
-
-
 Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
2.6.1.9 0°C, 1 month, 30% loss of activity Bacillus subtilis
2.6.1.57 0°C, 1 month, 15% loss of activity Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.9 L-histidinol phosphate + 2-oxoglutarate
-
 Bacillus subtilis 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu
-
 ?
2.6.1.9 L-phenylalanine + 2-oxoglutarate
-
 Bacillus subtilis phenylpyruvate + L-glutamate
-
 ?
2.6.1.9 L-tryptophan + 2-oxoglutarate
-
 Bacillus subtilis 3-indole-2-oxopropanoate + L-glutamate
-
 ?
2.6.1.9 L-tyrosine + 2-oxoglutarate
-
 Bacillus subtilis 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 ?
2.6.1.57 L-phenylalanine + 2-oxoglutarate
-
 Bacillus subtilis phenylpyruvate + L-glutamate
-
 ?
2.6.1.57 L-tryptophan + 2-oxoglutarate
-
 Bacillus subtilis 3-indole-2-oxopropanoate + L-glutamate
-
 ?
2.6.1.57 L-tyrosine + 2-oxoglutarate
-
 Bacillus subtilis 4-hydroxyphenylpyruvate + L-glutamate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.9 55
-
 4 min, more than 90% loss of activity, 10 min, in presence of pyridoxal 5'-phosphate, 75% loss of activity Bacillus subtilis
2.6.1.57 additional information
-
 2-oxoglutarate or pyridoxal 5'-phosphate stabilize against heat inactivation Bacillus subtilis
2.6.1.57 55
-
 10 min, 90% loss of activity without stabilizing agent, 10% loss of activity in the presence of 0.6 mM pyridoxal 5'-phosphate and 1 mM 2-oxoglutarate Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.9 7.1
-
-
 Bacillus subtilis
2.6.1.57 7.3
-
-
 Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.9 6 8.5 stable overnight Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.9 pyridoxal 5'-phosphate a pyridoxal phosphate protein Bacillus subtilis
2.6.1.9 pyridoxal 5'-phosphate 0.0022 mM Bacillus subtilis
2.6.1.57 pyridoxal 5'-phosphate Km: 0.025 mM Bacillus subtilis