Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mavrides, C.
    Transamination of aromatic amino acids in Escherichia coli (1987), Methods Enzymol., 142, 253-267.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.57 stable to freezing and thawing in phosphate buffers supplemented with pyridoxal phosphate, 2-oxoglutarate, dithiothreitol and EDTA Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.57 0.33
-
 L-phenylalanine pH 7.3, 37°C Escherichia coli
2.6.1.57 0.62
-
 L-tyrosine pH 7.3, 37°C Escherichia coli
2.6.1.57 2.5
-
 2-oxoglutarate pH 7.3, 37°C Escherichia coli
2.6.1.57 3.13
-
 oxaloacetate pH 7.3, 37°C Escherichia coli
2.6.1.57 10
-
 L-tryptophan pH 7.3, 37°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.57 88000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.57 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.57 ammonium sulfate, 55°C, 5 min, calcium phosphate gel, DEAE-cellulose, isoelectric focusing Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.57 70.2
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.6.1.57 -25°C, protein concentration: 0.03-0.2 mg/ml, potassium phosphate pH 7.0, 1 mM EDTA, 1 mM dithiothreitol, 2 mM 2-oxoglutarate and 0.2 mM pyridoxal 5'-phosphate, several months, no loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.57 3-iodo-L-tyrosine + 2-oxoglutarate
-
 Escherichia coli 3-(3-iodophenyl)-2-oxopropanoate + L-glutamate
-
 ?
2.6.1.57 3-iodo-L-tyrosine + oxaloacetate
-
 Escherichia coli 3-(3-iodophenyl)-2-oxopropanoate + L-aspartate
-
 ?
2.6.1.57 aspartate + 2-oxoglutarate weak activity Escherichia coli oxaloacetate + L-glutamate
-
 r
2.6.1.57 L-leucine + 2-oxoglutarate weak activity Escherichia coli 4-methyl-2-oxopentanoate + L-glutamate
-
 ?
2.6.1.57 L-methionine + 2-oxoglutarate weak activity Escherichia coli 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
 ?
2.6.1.57 L-phenylalanine + 2-oxoglutarate
-
 Escherichia coli phenylpyruvate + L-glutamate
-
 ?
2.6.1.57 L-phenylalanine + oxaloacetate
-
 Escherichia coli phenylpyruvate + L-aspartate
-
 ?
2.6.1.57 L-tryptophan + 2-oxoglutarate weak activity Escherichia coli 3-indole-2-oxopropanoate + L-glutamate
-
 r
2.6.1.57 L-tryptophan + oxaloacetate
-
 Escherichia coli 3-indole-2-oxopropanoate + L-aspartate
-
 ?
2.6.1.57 L-tyrosine + 2-oxoglutarate
-
 Escherichia coli p-hydroxyphenylpyruvate + L-glutamate
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.57 55
-
 5% loss of activity after 5 min in the presence of 0.2 mM pyridoxal 5'-phosphate and 2 mM 2-oxoglutarate, 60% loss of activity in the absence of cofactor and substrate after 5 min, more than 90% after 10 min Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.57 152
-
 L-phenylalanine pH 7.3, 37°C Escherichia coli
2.6.1.57 250
-
 L-tryptophan pH 7.3, 37°C Escherichia coli
2.6.1.57 278
-
 2-oxoglutarate pH 7.3, 37°C Escherichia coli
2.6.1.57 333
-
 L-tyrosine pH 7.3, 37°C Escherichia coli
2.6.1.57 500
-
 oxaloacetate pH 7.3, 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.57 7.5
-
 tryptophan Escherichia coli
2.6.1.57 8
-
 with tyrosine Escherichia coli
2.6.1.57 8
-
 with phenylalanine Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.57 pyridoxal 5'-phosphate a pyridoxal phosphate protein Escherichia coli