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Literature summary extracted from

  • Buchli, R.; Alberati-Giani, D.; Malherbe, P.; Kohler, C.; Broger, C.; Cesura, A.M.
    Cloning and functional expression of a soluble form of kynurenine/alpha-aminoadipate aminotransferase from rat kidney (1995), J. Biol. Chem., 270, 29330-29335.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.6.1.39 medicine L-2-aminoadipate, the substrate for AadAT, is a well known astroglial-specific toxin, knowledge of the cerebral disposition of this compound is instrumental for the elucidation of its mechanism of toxicity and possible relevance in pathology Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.7 transient expression in HEK-293 cells Rattus norvegicus
2.6.1.39 kynurenine/alpha-aminoadipate aminotransferase cDNA cloned and expressed in HEK-293 cells Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.7 0.045
-
 2-oxoglutarate pH 8.0, 37°C, recombinant enzyme, cosubstrate L-kyrunenine Rattus norvegicus
2.6.1.7 0.95
-
 L-kynurenine pH 8.0, 37°C, recombinant enzyme Rattus norvegicus
2.6.1.7 1.36
-
 L-3-hydroxykynurenine pH 8.0, 37°C, recombinant enzyme Rattus norvegicus
2.6.1.7 5.6
-
 L-glutamate pH 8.0, 37°C, alpha-aminoadipate aminotransferase activity Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.7 soluble
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.7 45000
-
 2 * 45000, SDS-PAGE Rattus norvegicus
2.6.1.7 90000 100000 gel filtration Rattus norvegicus
2.6.1.39 45000
-
 2 * 45000, SDS-PAGE Rattus norvegicus
2.6.1.39 47789
-
 2 * 47789, amino acid residues Rattus norvegicus
2.6.1.39 100000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.39 L-2-aminoadipate + 2-oxoglutarate Rattus norvegicus lysine catabolic pathway 2-oxoadipate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.7 Rattus norvegicus Q64602
-
-
2.6.1.39 Rattus norvegicus Q64602
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.7 ammonium sulfate, DEAE-Sepharose, phenyl-Sepharose, Mono Q, Mono P Rattus norvegicus
2.6.1.39
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.7 kidney
-
 Rattus norvegicus
-
2.6.1.39 kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.7 1.56
-
-
 Rattus norvegicus
2.6.1.39 1.56
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.7 L-3-hydroxykynurenine + 2-oxoglutarate
-
 Rattus norvegicus xanthurenic acid + L-glutamate
-
 ?
2.6.1.7 L-kynurenine + 2-oxoadipate 91% of activity with 2-oxoglutarate Rattus norvegicus kynurenic acid + 2-aminoadipate + H2O
-
 ?
2.6.1.7 L-kynurenine + 2-oxobutyrate 28% of activity with 2-oxoglutarate Rattus norvegicus kynurenic acid + 2-aminobutanoate + H2O
-
 ?
2.6.1.7 L-kynurenine + 2-oxoglutarate enzyme has both kynurenine aminotransferase and alpha-aminoadipate aminotransferase activity Rattus norvegicus kynurenic acid + L-glutamate + H2O
-
 ?
2.6.1.7 L-kynurenine + 4-methyl-2-oxovalerate 5.9% of activity with 2-oxoglutarate Rattus norvegicus kynurenic acid + 2,4-dimethylvalerate + H2O
-
 ?
2.6.1.7 L-kynurenine + phenylpyruvate 43% of activity with 2-oxoglutarate Rattus norvegicus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-phenylalanine
-
 ?
2.6.1.7 L-kynurenine + pyruvate 3% of activity with 2-oxoglutarate Rattus norvegicus kynurenic acid + L-alanine + H2O
-
 ?
2.6.1.39 kynurenine + 2-oxoglutarate
-
 Rattus norvegicus kynurenic acid + L-glutamate
-
 ir
2.6.1.39 L-2-aminoadipate + 2-oxoglutarate
-
 Rattus norvegicus 2-oxoadipate + L-glutamate
-
 r
2.6.1.39 L-2-aminoadipate + 2-oxoglutarate lysine catabolic pathway Rattus norvegicus 2-oxoadipate + L-glutamate
-
 r
2.6.1.39 additional information 2-aminoadipate aminotransferase and kynurenine aminotransferase, EC 2.6.1.7, activities are properties of a single protein Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.7 dimer 2 * 45000, SDS-PAGE Rattus norvegicus
2.6.1.39 dimer 2 * 45000, SDS-PAGE Rattus norvegicus
2.6.1.39 dimer 2 * 47789, amino acid residues Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.6.1.7 KAT
-
 Rattus norvegicus
2.6.1.39 AadAT
-
 Rattus norvegicus
2.6.1.39 kynurenine/alpha-aminoadipate aminotransferase
-
 Rattus norvegicus