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Literature summary extracted from

  • Tobes, M.C.
    Kynurenine-oxoglutarate aminotransferase from rat kidney (1987), Methods Enzymol., 142, 217-224.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.7 2-mercaptoethanol activation Rattus norvegicus

General Stability

EC Number General Stability Organism
2.6.1.7 dialysis of the apoenzyme against Tris-HCl or imidazole-HCl buffer inactivates Rattus norvegicus
2.6.1.7 stable in phosphate buffer supplemented with pyridoxal phosphate Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.7 2-Aminoadipate
-
Rattus norvegicus
2.6.1.7 2-oxoadipate
-
Rattus norvegicus
2.6.1.7 2-oxoglutarate substrate inhibition Rattus norvegicus
2.6.1.7 adipate
-
Rattus norvegicus
2.6.1.7 Co2+
-
Rattus norvegicus
2.6.1.7 Dicarboxylic acids C-2 to C-10 Rattus norvegicus
2.6.1.7 Hg2+
-
Rattus norvegicus
2.6.1.7 hydroxylamine complete inhibition Rattus norvegicus
2.6.1.7 KCN complete inhibition Rattus norvegicus
2.6.1.7 L-kynurenine substrate inhibition above 6 mM Rattus norvegicus
2.6.1.7 additional information not inhibited by L-3-hydroxykynurenine at higher concentrations Rattus norvegicus
2.6.1.7 Ni2+
-
Rattus norvegicus
2.6.1.7 phosphate
-
Rattus norvegicus
2.6.1.7 Sn2+
-
Rattus norvegicus
2.6.1.7 sulfate
-
Rattus norvegicus
2.6.1.7 Sulfate or phosphate esters of steroids
-
Rattus norvegicus
2.6.1.7 Zn2+
-
Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.7 0.00085
-
pyridoxal 5'-phosphate pH 6.5, 37°C Rattus norvegicus
2.6.1.7 1
-
2-oxoglutarate pH 6.5, 37°C Rattus norvegicus
2.6.1.7 1.4
-
L-kynurenine pH 6.5, 37°C Rattus norvegicus
2.6.1.7 2
-
L-3-hydroxykynurenine pH 6.5, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.7 mitochondrion
-
Rattus norvegicus 5739
-
2.6.1.7 soluble
-
Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.7 45000
-
2 * 45000, SDS-PAGE in the absence of 2-mercaptoethanol Rattus norvegicus
2.6.1.7 45500
-
2 * 45500, SDS-PAGE in the presence of 2-mercaptoethanol Rattus norvegicus
2.6.1.7 85000
-
gel filtration, sucrose density gradient centrifugation Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.7 L-kynurenine + 2-oxoglutarate Rattus norvegicus inducible enzyme of tryptophan catabolism kynurenic acid + L-glutamate + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.7 Rattus norvegicus
-
adult albino
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.7 acid precipitation, ammonium sulfate, DEAE-cellulose, hydroxylapatite Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.7 L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate transamination of the amino acid to a transient keto acid intermediate which spontaneously undergoes ring closure Rattus norvegicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.7 2.65
-
-
Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.7 3,5-diiodo-L-tyrosine + 2-oxoglutarate slightly better than L-kynurenine Rattus norvegicus 3-(2,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
?
2.6.1.7 L-2-aminoheptane-1,7-dioate + 2-oxoglutarate at 39.7% of the activity with 2-aminohexane-1,6-dioate Rattus norvegicus 2-oxoheptane-1,7-dioate + L-glutamate
-
?
2.6.1.7 L-3-hydroxykynurenine + 2-oxoglutarate no activity with D-isomer Rattus norvegicus xanthurenic acid + L-glutamate
-
?
2.6.1.7 L-kynurenine + 2-oxoglutarate
-
Rattus norvegicus kynurenic acid + L-glutamate + H2O
-
?
2.6.1.7 L-kynurenine + 2-oxoglutarate inducible enzyme of tryptophan catabolism Rattus norvegicus kynurenic acid + L-glutamate + H2O
-
?
2.6.1.7 L-kynurenine + 2-oxohexanedioate
-
Rattus norvegicus kynurenic acid + 2-aminohexanedioate + H2O
-
?
2.6.1.7 L-kynurenine + pyruvate less effective than 2-oxoglutarate Rattus norvegicus kynurenic acid + L-alanine + H2O
-
?
2.6.1.7 L-methionine + 2-oxoglutarate 12% the rate of L-kynurenine Rattus norvegicus 4-methylsulfanyl-2-oxobutanoate + glutamate
-
?
2.6.1.7 L-norleucine + 2-oxoglutarate at about 30% the rate of L-kynurenine Rattus norvegicus 2-oxohexanoate + L-glutamate
-
?
2.6.1.7 L-phenylalanine + 2-oxoglutarate
-
Rattus norvegicus phenylpyruvate + L-glutamate
-
?
2.6.1.7 L-tryptophan + 2-oxoglutarate 16% the rate of L-kynurenine Rattus norvegicus 3-indole-2-oxopropanoate + L-glutamate
-
?
2.6.1.7 L-tyrosine + 2-oxoglutarate
-
Rattus norvegicus 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
?

Subunits

EC Number Subunits Comment Organism
2.6.1.7 dimer 2 * 45500, SDS-PAGE in the presence of 2-mercaptoethanol Rattus norvegicus
2.6.1.7 dimer 2 * 45000, SDS-PAGE in the absence of 2-mercaptoethanol Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.7 37
-
assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.7 40
-
and above, 5 min, decreasing activity Rattus norvegicus
2.6.1.7 70
-
5 min, complete inactivation Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.7 additional information
-
pI: 6.56 Rattus norvegicus
2.6.1.7 6.5
-
with L-kynurenine as substrate Rattus norvegicus
2.6.1.7 7.5
-
with L-alpha-aminoadipate as substrate Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.7 5
-
and below, inactivation even in the presence of pyridoxal phosphate Rattus norvegicus
2.6.1.7 6.5 7.5 most stable Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.7 pyridoxal 5'-phosphate Km-value: 0.00085 mM Rattus norvegicus