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Literature summary extracted from

  • Stadtman, T.C.
    Clostridial glycine reductase: protein C, the acetyl group acceptor, catalyzes the arsenate-dependent decomposition of acetyl phosphate (1989), Proc. Natl. Acad. Sci. USA, 86, 7853-7856.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.21.4.2 arsenate absolutely required for protein C activity Acetoanaerobium sticklandii
1.21.4.2 thiols such as dithiothreitol required for decomposition of acetyl phosphate by protein C Acetoanaerobium sticklandii

General Stability

EC Number General Stability Organism
1.21.4.2 the ability of protein component C to catalyse the arsenate-dependent decomposition of acetyl phosphate is inhibited by alkylation selenoprotein A alkylated at pH 6 with bromoacetate is active as a component of the enzyme complex Acetoanaerobium sticklandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.4.2 Mg2+ required for decomposition of acetyl phosphate by protein C Acetoanaerobium sticklandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.21.4.2 additional information
-
 molecular mass is depending on the salt concentration present Acetoanaerobium sticklandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.4.2 acetyl phosphate + NH3 + thioredoxin disulfide + H2O Acetoanaerobium sticklandii
-
 glycine + phosphate + thioredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.4.2 Acetoanaerobium sticklandii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.4.2 of protein C of enzyme Acetoanaerobium sticklandii

Reaction

EC Number Reaction Comment Organism Reaction ID
1.21.4.2 acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin the protein C component catalyses the arsenate-dependent decomposition of acetyl phosphate Acetoanaerobium sticklandii
a
1.21.4.2 acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin the protein component C serves as the acetyl group acceptor in the overall reaction Acetoanaerobium sticklandii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.4.2 acetyl phosphate + NH3 + thioredoxin disulfide + H2O
-
 Acetoanaerobium sticklandii glycine + phosphate + thioredoxin
-
 ?
1.21.4.2 acetyl phosphate + NH3 + thioredoxin disulfide + H2O reduction of glycine action is coupled with formation of ATP from ADP Acetoanaerobium sticklandii glycine + phosphate + thioredoxin
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.21.4.2 62 64 protein C inactivated Acetoanaerobium sticklandii