EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.21.4.2 | (NH4)2SO4 | - |
Acetoanaerobium sticklandii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.21.4.2 | KBH4 | inactivates protein B, very little effect on fraction C | Acetoanaerobium sticklandii | |
1.21.4.2 | LiCl | - |
Acetoanaerobium sticklandii | |
1.21.4.2 | additional information | no effect: CaCl2, CoCl2, MnCl2 | Acetoanaerobium sticklandii | |
1.21.4.2 | NaCl | - |
Acetoanaerobium sticklandii | |
1.21.4.2 | NH2OH | inhibits protein B and fraction C | Acetoanaerobium sticklandii | |
1.21.4.2 | NH4Cl | - |
Acetoanaerobium sticklandii | |
1.21.4.2 | Zn(COOH)2 | - |
Acetoanaerobium sticklandii | |
1.21.4.2 | ZnCl2 | - |
Acetoanaerobium sticklandii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.21.4.2 | acetyl phosphate + NH3 + thioredoxin disulfide + H2O | Acetoanaerobium sticklandii | - |
glycine + phosphate + thioredoxin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.21.4.2 | Acetoanaerobium sticklandii | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.21.4.2 | acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin | mechanism | Acetoanaerobium sticklandii | |
1.21.4.2 | acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin | The reaction is observed only in the direction of glycine reduction. The enzyme consists of three protein components A, B and C. Protein B contains selenocysteine and a pyruvoyl group, and is responsible for glycine binding and ammonia release. Protein A, which also contains selenocysteine, is reduced by thioredoxin, and is needed to convert the carboxymethyl group into a ketene equivalent, in turn used by protein C to produce acetyl phosphate. Only protein B distinguishes this enzyme from EC 1.21.4.3 (sarcosine reductase) and EC 1.21.4.4 (betaine reductase) | Acetoanaerobium sticklandii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.21.4.2 | 5.5 | - |
protein B of enzyme | Acetoanaerobium sticklandii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.21.4.2 | acetyl phosphate + NH3 + thioredoxin disulfide + H2O | - |
Acetoanaerobium sticklandii | glycine + phosphate + thioredoxin | - |
? | |
1.21.4.2 | acetyl phosphate + NH3 + thioredoxin disulfide + H2O | reduction of glycine action is coupled with formation of ATP from ADP | Acetoanaerobium sticklandii | glycine + phosphate + thioredoxin | - |
? |