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Literature summary extracted from

  • Goodenough, P.W.; Kessell, S.; Lea, A.G.H.; Loeffler, T.
    Mono- and diphenolase activity from fruit of Malus pumila (1983), Phytochemistry, 22, 359-363.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.18.1 L-Dopa and analogs, activation Malus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 O2 at concentrations above 30% Malus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 additional information
-
 additional information 4.3% O2 Malus sp.
1.14.18.1 0.2
-
 caffeic acid
-
 Malus sp.
1.14.18.1 0.2
-
 3-hydroxyphloridzin
-
 Malus sp.
1.14.18.1 2.7
-
 L-Dopa
-
 Malus sp.
1.14.18.1 3.2
-
 4-methylcatechol
-
 Malus sp.
1.14.18.1 4
-
 p-coumaric acid
-
 Malus sp.
1.14.18.1 4.7
-
 catechin
-
 Malus sp.
1.14.18.1 5.7
-
 epicatechin
-
 Malus sp.
1.14.18.1 5.9
-
 chlorogenic acid
-
 Malus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 26000
-
 x * 26000, SDS-PAGE Malus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Malus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1
-
 Malus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 fruit
-
 Malus sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 3-hydroxyphloridzin + O2
-
 Malus sp. ?
-
 ?
1.14.18.1 4-methylcatechol + O2
-
 Malus sp. 4-methyl-o-benzoquinone + H2O
-
 ?
1.14.18.1 caffeic acid + 1/2 O2
-
 Malus sp. caffeoyl quinone + H2O
-
 ?
1.14.18.1 catechin + O2
-
 Malus sp. catechin-O-quinone + H2O
-
 r
1.14.18.1 chlorogenic acid + O2
-
 Malus sp. chlorogenoquinone + H2O
-
 ?
1.14.18.1 D-catechin + O2
-
 Malus sp. ?
-
 ?
1.14.18.1 epicatechin + O2
-
 Malus sp. ?
-
 ?
1.14.18.1 L-DOPA + O2
-
 Malus sp. dopaquinone + H2O
-
 r
1.14.18.1 L-epicatechin + O2
-
 Malus sp. ?
-
 ?
1.14.18.1 p-coumaric acid + O2 + AH2
-
 Malus sp. caffeic acid + H2O + A
-
 ?
1.14.18.1 phloridzin + O2
-
 Malus sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.1 ? x * 26000, SDS-PAGE Malus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 4.5
-
 minimal activity at pH 5.5 Malus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.18.1 5.5
-
 no activity below Malus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.18.1 3.5 4.5 optimal stability at pH 4.5, less stable above pH 6.0 Malus sp.
1.14.18.1 6
-
 4 days at 2°C, 70% inactivation Malus sp.