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Literature summary extracted from

  • Wichers, H.J.; Peetsma, G.J.; Malingre, T.M.; Huizing, H.J.
    Purification and properties of a phenol oxidase derived from suspension cultures of Mucuna pruriens (1984), Planta, 162, 334-341.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.18.1 ascorbate activation of tyrosine hydroxylase activity Mucuna pruriens

General Stability

EC Number General Stability Organism
1.14.18.1 tyrosine hydroxylation activity is inactivated faster than catecholase activity Mucuna pruriens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 azide 1 mM, 60% inhibition of catecholase activity Mucuna pruriens
1.14.18.1 diethyldithiocarbamate 0.05 mM, 97% inhibition of tyrosinase activity, 83% inhibition of catecholase activity Mucuna pruriens
1.14.18.1 L-ascorbic acid
-
 Mucuna pruriens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 0.18
-
 tyramine
-
 Mucuna pruriens
1.14.18.1 1.09
-
 dopamine
-
 Mucuna pruriens
1.14.18.1 1.45
-
 Adrenaline bitartrate
-
 Mucuna pruriens
1.14.18.1 1.8
-
 L-tyrosine
-
 Mucuna pruriens
1.14.18.1 2
-
 4-[(1R)-2-amino-1-hydroxyethyl]benzene-1,2-diol
-
 Mucuna pruriens
1.14.18.1 2.5
-
 alpha-Methyl-dopa
-
 Mucuna pruriens
1.14.18.1 5.55
-
 D-Dopa
-
 Mucuna pruriens
1.14.18.1 6.66
-
 L-Dopa
-
 Mucuna pruriens
1.14.18.1 8
-
 N-Formyl-L-tyrosine
-
 Mucuna pruriens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 42000
-
 2 * 42000, SDS-PAGE Mucuna pruriens
1.14.18.1 90000
-
 gel filtration Mucuna pruriens

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Mucuna pruriens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 ammonium sulfate, Celite chromatography, DEAE-Sepharose Mucuna pruriens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 cell culture
-
 Mucuna pruriens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 658
-
 L-dopa oxidation Mucuna pruriens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 adrenaline bitartrate + O2
-
 Mucuna pruriens ?
-
 ?
1.14.18.1 alpha-methyl-dopa + O2
-
 Mucuna pruriens alpha-methyldopaquinone + H2O
-
 ?
1.14.18.1 D-dopa + O2
-
 Mucuna pruriens dopaquinone + H2O
-
 r
1.14.18.1 dopa + 1/2 O2 L-dopa Mucuna pruriens dopaquinone + H2O
-
 ?
1.14.18.1 dopa + 1/2 O2 D-dopa Mucuna pruriens dopaquinone + H2O
-
 ?
1.14.18.1 dopamine + 1/2 O2
-
 Mucuna pruriens dopamine quinone + H2O
-
 ?
1.14.18.1 L-tyrosine + O2 + AH2
-
 Mucuna pruriens L-dopa + H2O + A
-
 ?
1.14.18.1 N-formyl-L-tyrosine + O2
-
 Mucuna pruriens ?
-
 ?
1.14.18.1 noradrenaline + O2
-
 Mucuna pruriens ?
-
 ?
1.14.18.1 tyramine + O2
-
 Mucuna pruriens 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.1 dimer 2 * 42000, SDS-PAGE Mucuna pruriens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 5.5 6.5 catecholase activity Mucuna pruriens
1.14.18.1 7.5
-
 tyrosine hydroxylase Mucuna pruriens