Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Birse, C.E.; Clutterbuck, A.J.
    N-acetyl-6-hydroxytryptophan oxidase, a developmentally controlled phenol oxidase from Aspergillus nidulans (1990), J. Gen. Microbiol., 136, 1725-1730.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 1-Phenyl-2-thiourea 1 mM, 94% inhibition Aspergillus nidulans
1.14.18.1 8-hydroxyquinoline 3 mM, 44% inhibition Aspergillus nidulans
1.14.18.1 azide no inhibition by sodium azide Aspergillus nidulans
1.14.18.1 diethyldithiocarbamate 0.1 mM, complete inhibition Aspergillus nidulans
1.14.18.1 Metabisulfite 0.1 mM, 99% inhibition Aspergillus nidulans
1.14.18.1 o-phenanthroline hydrate 3 mM, 30% inhibition Aspergillus nidulans
1.14.18.1 phenylhydrazine 1 mM, 86% inhibition, noncompetitive inhibition Aspergillus nidulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 2 14 Hydroquinone monomethylether range of estimated values for crude or purified extracts Aspergillus nidulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.1 extracellular
-
 Aspergillus nidulans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.1 copper 2.1 copper atoms per polypeptide, enzyme form B Aspergillus nidulans
1.14.18.1 Zn2+ 0.9 mol per polypeptide Aspergillus nidulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 48000
-
 x * 48000, enzyme form B, SDS-PAGE Aspergillus nidulans
1.14.18.1 50000
-
 x * 50000, enzyme form A, SDS-PAGE Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.1 N-acetyl-6-hydroxytryptophan + O2 Aspergillus nidulans
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Aspergillus nidulans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 DEAE-Sepharose, Phenyl Sepharose, enzyme form A and B Aspergillus nidulans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 conidiophore
-
 Aspergillus nidulans
-
1.14.18.1 culture filtrate
-
 Aspergillus nidulans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 additional information
-
 enzyme form A, 480.0 DELTA absorbance 470 nm/min/mg, enzyme form B, 258.6 DELTA absorbance 470 nm/min/mg Aspergillus nidulans

Storage Stability

EC Number Storage Stability Organism
1.14.18.1 4°C, 3 weeks, no loss of activity Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 4-methylcatechol + O2
-
 Aspergillus nidulans 4-methyl-o-benzoquinone + H2O
-
 ?
1.14.18.1 catechol + 1/2 O2
-
 Aspergillus nidulans 1,2-benzoquinone + H2O
-
 ?
1.14.18.1 chlorogenic acid + O2
-
 Aspergillus nidulans chlorogenoquinone + H2O
-
 ?
1.14.18.1 D-catechin + O2
-
 Aspergillus nidulans ?
-
 ?
1.14.18.1 hydroquinone monomethylether + O2
-
 Aspergillus nidulans quinone monomethylether
-
 ?
1.14.18.1 N-acetyl-6-hydroxytryptophan + O2
-
 Aspergillus nidulans ?
-
 ?
1.14.18.1 o-methoxyphenol + O2 + AH2 trivial name guaiacol Aspergillus nidulans 1,2-dihydroxy-3-methoxybenzene + H2O + A
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.1 ? x * 48000, enzyme form B, SDS-PAGE Aspergillus nidulans
1.14.18.1 ? x * 50000, enzyme form A, SDS-PAGE Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 7
-
 with hydroquinone monomethylether Aspergillus nidulans