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Literature summary extracted from

  • Wong, Y.S.; Castelfranco, P.A.
    Resolution and reconstitution of magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase, the enzyme system responsible for the formation of the chlorophyll isocyclic ring (1984), Plant Physiol., 75, 658-661.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.81 ATP requirement for reconstituted system Cucumis sativus
1.14.13.81 S-adenosyl-L-methionine 1 mM, absolute requirement when magnesium-protoporphyrin IX is used as substrate Cucumis sativus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.81 methyl viologen at micromolar concentrations Cucumis sativus
1.14.13.81 methylene blue at micromolar concentrations Cucumis sativus
1.14.13.81 phenazine methosulfate at micromolar concentrations Cucumis sativus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.81 chloroplast
-
 Cucumis sativus 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.81 magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 Cucumis sativus
-
 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.81 Cucumis sativus
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.14.13.81 reconstitution of the enzyme system from a high-speed supernatant and a membrane pellet Cucumis sativus

Storage Stability

EC Number Storage Stability Organism
1.14.13.81 -15°C, 30% loss of activity in supernatant, 70% loss of activity in pellet after 7 days Cucumis sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.81 magnesium-protoporphyrin IX + NADPH + O2
-
 Cucumis sativus ? + NADP+ + H2O
-
 ?
1.14.13.81 magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2
-
 Cucumis sativus 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O
-
 ?
1.14.13.81 additional information overview: tetrapyrrole with substituents at various positions Cucumis sativus ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.81 NADH
-
 Cucumis sativus
1.14.13.81 NADPH more effective than NADH below 2 mM, equally effective at 10 mM Cucumis sativus