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Literature summary extracted from
- Glycerol dehydrogenase from Aerobacter aerogenes (1955), Methods Enzymol., 1, 397-400.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.6 | NH4+ | - |
Klebsiella aerogenes |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.6 | 0.014 | - |
NADH | - |
Klebsiella aerogenes | |
| 1.1.1.6 | 0.15 | - |
NAD+ | - |
Klebsiella aerogenes | |
| 1.1.1.6 | 1.3 | - |
dihydroxyacetone | - |
Klebsiella aerogenes | |
| 1.1.1.6 | 39 | - |
glycerol | - |
Klebsiella aerogenes | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.1.6 | cytoplasm | soluble, isolation from crude cell extract | Klebsiella aerogenes | 5737 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.6 | glycerol + NAD+ | Klebsiella aerogenes | - |
dihydroxyacetone + NADH + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.6 | Klebsiella aerogenes | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.6 | - |
Klebsiella aerogenes |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.6 | 82 | - |
- |
Klebsiella aerogenes |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.6 | frozen, retains about 42% of initial activity for 2 months | Klebsiella aerogenes |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.6 | 1,2-propanediol + NAD+ | as active as glycerol | Klebsiella aerogenes | hydroxyacetone + NADH | - |
? | |
| 1.1.1.6 | 1,3-propanediol + NAD+ | relative rate of oxidation is 37% compared to oxidation of glycerol | Klebsiella aerogenes | ? | - |
? | |
| 1.1.1.6 | 1,4-butanediol + NAD+ | relative activity 17% compared to oxidation of glycerol | Klebsiella aerogenes | ? | - |
? | |
| 1.1.1.6 | 2,3-butanediol + NAD+ | as active as glycerol | Klebsiella aerogenes | 3-hydroxybutane-2-one + NADH | - |
? | |
| 1.1.1.6 | beta-glycerophosphate + NAD+ | relative activity is 2% compared to oxidation of glycerol | Klebsiella aerogenes | ? | - |
? | |
| 1.1.1.6 | diglycerol + NAD+ | relative activity is 21% compared to oxidation of glycerol | Klebsiella aerogenes | ? | - |
? | |
| 1.1.1.6 | DL-alpha-glycerophosphate + NAD+ | relative activity 11%compared to oxidation of glycerol | Klebsiella aerogenes | ? | - |
? | |
| 1.1.1.6 | DL-glyceraldehyde + NAD+ | relative rate of reduction is 14%compared to reduction of dihydroxyacetone | Klebsiella aerogenes | 3-hydroxypyruvaldehyde + NADH | - |
? | |
| 1.1.1.6 | ethanol + NAD+ | relative activity is 1% compared to oxidation of glycerol | Klebsiella aerogenes | acetaldehyde + NADH | - |
? | |
| 1.1.1.6 | ethylene glycol + NAD+ | relative activity is 20% compared to oxidation of glycerol | Klebsiella aerogenes | ? | - |
? | |
| 1.1.1.6 | glycerol + NAD+ | - |
Klebsiella aerogenes | dihydroxyacetone + NADH + H+ | - |
? | |
| 1.1.1.6 | glycerol-alpha-monochlorohydrin + NAD+ | relative rate of oxidation is 26% compared to oxidation of glycerol | Klebsiella aerogenes | ? | - |
? | |
| 1.1.1.6 | hydroxy-2-propanone + NADH | relative activity is 27% compared to oxidation of glycerol | Klebsiella aerogenes | propylene glycol + NAD+ | - |
? | |
| 1.1.1.6 | i-inositol + NAD+ | relative activity is 18% compared to oxidation of glycerol | Klebsiella aerogenes | ? | - |
? | |
| 1.1.1.6 | isopropanol + NAD+ | relative activity is 17% compared to oxidation of glycerol | Klebsiella aerogenes | acetone + NADH | - |
? | |
| 1.1.1.6 | methylglyoxal + NADH | relative rate of reduction is 56% compared to reduction of dihydroxyacetone | Klebsiella aerogenes | lactaldehyde + NAD+ | - |
? | |
| 1.1.1.6 | sorbitol + NAD+ | relative activity is 3% compared to oxidation of glycerol | Klebsiella aerogenes | ? | - |
? | |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.6 | 6.8 | 11 | the initial rate of reaction increases upon increasing the pH from pH 6.8 to 11.0, past pH 11.0 the initial rate falls rapidly, oxidation of glycerol | Klebsiella aerogenes |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.6 | 4 | - |
25°C, 10 min, 30% loss of activity | Klebsiella aerogenes |
| 1.1.1.6 | 7.5 | - |
25°C, 10 min, maximal stability, 10% loss of activity | Klebsiella aerogenes |
| 1.1.1.6 | 10 | - |
25°C, 10 min, 40% loss of activity | Klebsiella aerogenes |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.6 | NAD+ | - |
Klebsiella aerogenes | |
| 1.1.1.6 | NADH | - |
Klebsiella aerogenes | |
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Release 2023.1 (January 2023)
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