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Literature summary extracted from

  • Marshall, J.H.; May, J.W.; Sloan, J.
    Purification and properties of glycerol:NAD+ 2-oxidoreductase (glycerol dehydrogenase) from Schizosaccharomyces pombe (1985), J. Gen. Microbiol., 131, 1581-1588.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.6 iodoacetamide at 1 mM 140% of the activity without activator, at 10 mM 220% of the activity without activator Schizosaccharomyces pombe
1.1.1.6 N-ethylmaleimide at 0.1 mM 145% of the activity without activator Schizosaccharomyces pombe
1.1.1.6 NH4+
-
Schizosaccharomyces pombe
1.1.1.6 p-chloromercuribenzoic acid at 0.1 mM 133% of the activity without activator Schizosaccharomyces pombe

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.6 2-mercaptoethanol
-
Schizosaccharomyces pombe
1.1.1.6 dithiothreitol
-
Schizosaccharomyces pombe
1.1.1.6 EDTA 10 mM Schizosaccharomyces pombe
1.1.1.6 L-cysteine
-
Schizosaccharomyces pombe
1.1.1.6 Li+ competitive, forward reaction Schizosaccharomyces pombe
1.1.1.6 Na+ competitive, forward reaction Schizosaccharomyces pombe
1.1.1.6 NADP+ inhibits oxidation of glycerol Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.6 0.06
-
dihydroxyacetone pH 6.0 Schizosaccharomyces pombe
1.1.1.6 0.12
-
NADH pH 6.0 Schizosaccharomyces pombe
1.1.1.6 0.13
-
NAD+ pH 10.0 Schizosaccharomyces pombe
1.1.1.6 0.5
-
glycerol pH 10.0 Schizosaccharomyces pombe

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.6 cytoplasm soluble, isolation from crude cell extract Schizosaccharomyces pombe 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.6 K+
-
Schizosaccharomyces pombe
1.1.1.6 Li+
-
Schizosaccharomyces pombe
1.1.1.6 Mn2+ increases activity significantly Schizosaccharomyces pombe
1.1.1.6 Zn2+ increases activity Schizosaccharomyces pombe

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.6 47000
-
8 * 47000, SDS-PAGE Schizosaccharomyces pombe
1.1.1.6 400000
-
gel filtration Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.6 glycerol + NAD+ Schizosaccharomyces pombe
-
dihydroxyacetone + NADH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.6 Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.6
-
Schizosaccharomyces pombe

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.6 9.3
-
-
Schizosaccharomyces pombe

Storage Stability

EC Number Storage Stability Organism
1.1.1.6 -70°C, long term storage Schizosaccharomyces pombe
1.1.1.6 4°C, in 2 M (NH4)2SO4, 1 M glycerol, several months Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.6 1,2,3-butanetriol + NAD+
-
Schizosaccharomyces pombe 1,3-dihydroxybutane-2-one + NADH
-
?
1.1.1.6 1,2-butanediol + NAD+
-
Schizosaccharomyces pombe 1-hydroxybutane-2-one + NADH
-
r
1.1.1.6 1,2-propanediol + NAD+
-
Schizosaccharomyces pombe hydroxyacetone + NADH
-
r
1.1.1.6 2,3-butanediol + NAD+
-
Schizosaccharomyces pombe 3-hydroxybutane-2-one + NADH
-
r
1.1.1.6 DL-glyceraldehyde + NAD+
-
Schizosaccharomyces pombe 3-hydroxypyruvaldehyde + NADH
-
?
1.1.1.6 ethanediol + NAD+
-
Schizosaccharomyces pombe glycolaldehyde + NADH
-
?
1.1.1.6 glycerol + NAD+
-
Schizosaccharomyces pombe dihydroxyacetone + NADH + H+
-
?
1.1.1.6 glycerol + NAD+
-
Schizosaccharomyces pombe dihydroxyacetone + NADH + H+
-
r
1.1.1.6 methylglyoxal + NADH
-
Schizosaccharomyces pombe lactaldehyde + NAD+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.6 octamer 8 * 47000, SDS-PAGE Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.6 5 6 reduction of dihydroxyacetone Schizosaccharomyces pombe
1.1.1.6 11 12 oxidation of glycerol Schizosaccharomyces pombe

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.6 5.5 9 pH 5.5: about 70% of maximal activity, pH 9.0: about 45% of maximal activity, reduction of dihydroxyacetone Schizosaccharomyces pombe
1.1.1.6 9 12 pH 9.0: about 45% of maximal activity, pH 11-12: maximal activity, oxidation of glycerol Schizosaccharomyces pombe

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.6 NAD+
-
Schizosaccharomyces pombe
1.1.1.6 NADH
-
Schizosaccharomyces pombe