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Literature summary extracted from

  • Hormann, K.; Andreesen, J.R.
    Reductive cleavage of sarcosine and betaine by Eubacterium acidaminophilum via enzyme systems different from glycine reductase (1989), Arch. Microbiol., 153, 50-59.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.21.4.3 dithioerythritol can serve as artificial electron donor Peptoclostridium acidaminophilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.21.4.3 hydroxylamine 20% inhibition at 1 mM Peptoclostridium acidaminophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.21.4.3 3.7
-
 N-methylglycine pH 7.7, 37°C Peptoclostridium acidaminophilum
1.21.4.3 11
-
 dithioerythritol pH 7.7, 37°C Peptoclostridium acidaminophilum
1.21.4.4 7.5
-
 dithioerythritol pH 7.7, 30°C Peptoclostridium acidaminophilum
1.21.4.4 9
-
 betaine pH 7.7, 30°C Peptoclostridium acidaminophilum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.21.4.3 cytoplasm
-
 Peptoclostridium acidaminophilum 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.4.3 N-methylglycine + phosphate + thioredoxin Peptoclostridium acidaminophilum involved in creatinine and creatine metabolism, intermediate of choline degradation acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
1.21.4.4 N,N,N-trimethylglycine + phosphate + thioredoxin Peptoclostridium acidaminophilum
-
 acetyl phosphate + trimethylamine + thioredoxin disulfide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.4.3 Peptoclostridium acidaminophilum
-
 ATCC 49065, DSM 3953, expression of enzyme is induced only when grown on sarcosine or creatine
-
1.21.4.4 Peptoclostridium acidaminophilum
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.21.4.3 137
-
 pH 7.7, 37°C, extract from bacteria that were grown on formate and creatine Peptoclostridium acidaminophilum
1.21.4.3 217
-
 pH 7.7, 37°C, extract from bacteria that were grown on formate and sarcosine Peptoclostridium acidaminophilum
1.21.4.4 0.117
-
-
 Peptoclostridium acidaminophilum

Storage Stability

EC Number Storage Stability Organism
1.21.4.3 -20°C, 15% loss of activity within one week Peptoclostridium acidaminophilum
1.21.4.4 -20°C, crude extract, stable Peptoclostridium acidaminophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.4.3 N-methylglycine + phosphate + dithioerythritol
-
 Peptoclostridium acidaminophilum acetyl phosphate + methylamine + dithioerythritol disulfide
-
 ?
1.21.4.3 N-methylglycine + phosphate + thioredoxin
-
 Peptoclostridium acidaminophilum acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
1.21.4.3 N-methylglycine + phosphate + thioredoxin involved in creatinine and creatine metabolism, intermediate of choline degradation Peptoclostridium acidaminophilum acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
1.21.4.4 N,N,N-trimethylglycine + dithioerythritol
-
 Peptoclostridium acidaminophilum ?
-
 ?
1.21.4.4 N,N,N-trimethylglycine + NADPH
-
 Peptoclostridium acidaminophilum ? + NADP+
-
 ?
1.21.4.4 N,N,N-trimethylglycine + phosphate + thioredoxin
-
 Peptoclostridium acidaminophilum acetyl phosphate + trimethylamine + thioredoxin disulfide
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.21.4.3 37
-
-
 Peptoclostridium acidaminophilum
1.21.4.4 37
-
-
 Peptoclostridium acidaminophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.21.4.3 7.7 8 highest activity in K-phosphate buffer Peptoclostridium acidaminophilum
1.21.4.4 7.7 8
-
 Peptoclostridium acidaminophilum

Cofactor

EC Number Cofactor Comment Organism Structure
1.21.4.3 NADH 5% of activity compared to NADPH Peptoclostridium acidaminophilum
1.21.4.3 NADPH preferred as physiological electron donor Peptoclostridium acidaminophilum
1.21.4.4 NADH
-
 Peptoclostridium acidaminophilum
1.21.4.4 NADPH preferred donor Peptoclostridium acidaminophilum