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Literature summary extracted from

  • Knobloch, K.H.; Hahlbrock, K.
    Isoenzymes of p-coumarate:CoA ligase from cell suspension cultures of Glycine max (1975), Eur. J. Biochem., 52, 311-320.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.12 4-coumarate above 0.1 mM Glycine max
6.2.1.12 CoASH above 0.1 mM Glycine max
6.2.1.12 Ionic strength activity of isoenzyme 1 decreases steadily with increasing ionic strength at least in the range from 0.1 to 0.8 M Tris-HCl, highest rate of isoenzyme 2 activity is obtained at concentrations of about 0.3 M Tris-HCl Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 0.004
-
 CoASH isoenzyme 2 Glycine max
6.2.1.12 0.007
-
 CoASH isoenzyme 1 Glycine max
6.2.1.12 0.009
-
 ferulate isoenzyme 1 Glycine max
6.2.1.12 0.011
-
 sinapoate isoenzyme 1 Glycine max
6.2.1.12 0.012
-
 5-hydroxyferulate isoenzyme 1 Glycine max
6.2.1.12 0.014
-
 caffeate isoenzyme 2 Glycine max
6.2.1.12 0.017
-
 4-coumarate isoenzyme 2 Glycine max
6.2.1.12 0.03
-
 3-coumarate isoenzyme 2 Glycine max
6.2.1.12 0.032
-
 4-coumarate isoenzyme 1 Glycine max
6.2.1.12 0.04
-
 caffeate isoenzyme 1 Glycine max
6.2.1.12 0.065
-
 ATP isoenzyme 2 Glycine max
6.2.1.12 0.09
-
 2-coumarate isoenzyme 2 Glycine max
6.2.1.12 0.1
-
 isoferulate 3,4-dimethoxycinnamate, , isoenzyme 1 Glycine max
6.2.1.12 0.12
-
 5-hydroxyferulate isoenzyme 2 Glycine max
6.2.1.12 0.13
-
 ferulate isoenzyme 2 Glycine max
6.2.1.12 0.15
-
 isoferulate isoenzyme 2 Glycine max
6.2.1.12 0.17
-
 ATP 3-coumarate, , isoenzyme 1 Glycine max
6.2.1.12 0.18
-
 2-coumarate isoenzyme 1 Glycine max
6.2.1.12 0.23
-
 3,4,5-trimethoxycinnamate isoenzyme 1 Glycine max
6.2.1.12 0.38
-
 4-methoxycinnamate isoenzyme 1 Glycine max
6.2.1.12 1.3
-
 cinnamate isoenzyme 1 Glycine max
6.2.1.12 4.5
-
 cinnamate isoenzyme 2 Glycine max

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.12 Co2+ divalent cation required, Mg2+, Mn2+ and Co2+ are equally effective Glycine max
6.2.1.12 Mg2+ divalent cation required, Mg2+, Mn2+ and Co2+ are equally effective Glycine max
6.2.1.12 Mn2+ divalent cation required, Mg2+, Mn2+ and Co2+ are equally effective Glycine max

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
6.2.1.12 Ethylene glycol 30% ethylene glycol stabilizes. Loss of activity after 7 days is reduced from 80% to about 25% Glycine max
6.2.1.12 Glycerol 30% glycerol stabilizes. Loss of activity after 7 days is reduced from 80% to about 25% Glycine max

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Glycine max
-
 var. Mandarin
-
6.2.1.12 Glycine max
-
 2 isoenzymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12 partial Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.12 cell culture
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.12 additional information
-
-
 Glycine max

Storage Stability

EC Number Storage Stability Organism
6.2.1.12 crude enzyme preparation is unstable in buffered aqueous solution at temperatures at or above 0°C Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 2-coumarate + CoA
-
 Glycine max AMP + diphosphate + 2-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 3,4,5-trimethoxycinnamate + CoA isoenzyme 1 is active, isoenzyme 2 not Glycine max AMP + diphosphate + 3,4,5-trimethoxycinnamoyl-CoA
-
 ?
6.2.1.12 ATP + 3,4-dimethoxycinnamate + CoA isoenzyme 1 is active, isoenzyme 2 not Glycine max AMP + diphosphate + 3,4-dimethoxycinnamoyl-CoA
-
 ?
6.2.1.12 ATP + 3-coumarate + CoA
-
 Glycine max AMP + diphosphate + 3-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA
-
 Glycine max AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-methoxycinnamate + CoA isoenzyme 1 is active, isoenzyme 2 not Glycine max AMP + diphosphate + 4-methoxycinnamoyl-CoA
-
 ?
6.2.1.12 ATP + 5-hydroxyferulate + CoA
-
 Glycine max AMP + diphosphate + 5-hydroxyferuloyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA
-
 Glycine max AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA
-
 Glycine max AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA
-
 Glycine max AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + isoferulate + CoA
-
 Glycine max AMP + diphosphate + isoferuloyl-CoA
-
 ?
6.2.1.12 ATP + sinapoate + CoA isoenzyme 1 is active with sinapoate, isoenzyme 2 not Glycine max AMP + diphosphate + sinapoyl-CoA
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.12 7.7
-
 formation of 4-coumaroyl-CoA, isoenzyme 2 Glycine max
6.2.1.12 8.3
-
 formation of 4-coumaroyl-CoA, isoenzyme 1 Glycine max