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Literature summary extracted from

  • Kato, Y.; Asano, Y.
    Purification and properties of crystalline 3-methylaspartase from two facultative anaerobes, Citrobacter sp. strain YG-0504 and Morganella morganii strain YG-0601 (1995), Biosci. Biotechnol. Biochem., 59, 93-99.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.1.2
-
Citrobacter freundii
4.3.1.2
-
Morganella morganii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.2 5,5'-dithiobis(2-nitrobenzoate) 1 mM, complete Citrobacter freundii
4.3.1.2 5,5'-dithiobis(2-nitrobenzoate) 1 mM 54%, loss of activity Morganella morganii
4.3.1.2 8-hydroxyquinoline 5 mM, 44% loss of activity Citrobacter freundii
4.3.1.2 8-hydroxyquinoline 5 mM, 88% loss of activity Morganella morganii
4.3.1.2 Ag+ complete Citrobacter freundii
4.3.1.2 Ag+ complete Morganella morganii
4.3.1.2 Al3+ 5 mM AlCl3, 95% loss of activity Morganella morganii
4.3.1.2 Ca2+ CaCl2 5 mM, 83% loss of activity Citrobacter freundii
4.3.1.2 Ca2+
-
Clostridium tetanomorphum
4.3.1.2 Ca2+ 5 mM CaCl2, 73% loss of activity Morganella morganii
4.3.1.2 Cd2+ 1 mM CdCl2, complete inhibition Morganella morganii
4.3.1.2 Co2+ 1 mM CoCl2, 60% loss of activity Morganella morganii
4.3.1.2 Cu2+ 1 mM CuSO4, 47% loss of activity Citrobacter freundii
4.3.1.2 Cu2+
-
Clostridium tetanomorphum
4.3.1.2 Cu2+ 1 mM CuSO4, 73% loss of activity Morganella morganii
4.3.1.2 Fe2+ 5 mM FeSO4, 56% loss of activity Citrobacter freundii
4.3.1.2 Fe2+ 5 mM FeSO4, 89% loss of activity Morganella morganii
4.3.1.2 Fe3+ 1 mM, 75% loss of activity Citrobacter freundii
4.3.1.2 Fe3+ 1 mM, 69% loss of activity Morganella morganii
4.3.1.2 Hg+ complete Citrobacter freundii
4.3.1.2 Hg+ complete Morganella morganii
4.3.1.2 Hg2+ complete Citrobacter freundii
4.3.1.2 Hg2+ complete Morganella morganii
4.3.1.2 iodoacetate 1 mM, 86% loss of activity Morganella morganii
4.3.1.2 additional information
-
Citrobacter freundii
4.3.1.2 additional information
-
Morganella morganii
4.3.1.2 N-ethylmaleimide complete Citrobacter freundii
4.3.1.2 N-ethylmaleimide
-
Clostridium tetanomorphum
4.3.1.2 N-ethylmaleimide complete Morganella morganii
4.3.1.2 p-chloromercuribenzoate complete Citrobacter freundii
4.3.1.2 p-chloromercuribenzoate complete Morganella morganii
4.3.1.2 Pb2+ 5 mM PbCl2, 88% loss of activity Morganella morganii
4.3.1.2 Sn2+ 1 mM SnCl2, 51% loss of activity Morganella morganii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.2 Cs+
-
Citrobacter freundii
4.3.1.2 Cs+
-
Morganella morganii
4.3.1.2 Li+
-
Citrobacter freundii
4.3.1.2 Li+
-
Morganella morganii
4.3.1.2 Mg2+
-
Citrobacter freundii
4.3.1.2 Mg2+
-
Clostridium tetanomorphum
4.3.1.2 Mg2+
-
Morganella morganii
4.3.1.2 Mn2+ activates Citrobacter freundii
4.3.1.2 Mn2+ activates Clostridium tetanomorphum
4.3.1.2 Mn2+ activates Morganella morganii
4.3.1.2 Na+
-
Citrobacter freundii
4.3.1.2 Na+
-
Morganella morganii
4.3.1.2 Ni2+ activates Citrobacter freundii
4.3.1.2 Ni2+ activates Clostridium tetanomorphum
4.3.1.2 Ni2+ activates Morganella morganii
4.3.1.2 Rb+
-
Citrobacter freundii
4.3.1.2 Rb+
-
Morganella morganii
4.3.1.2 Zn2+ activates Citrobacter freundii
4.3.1.2 Zn2+ activates Clostridium tetanomorphum
4.3.1.2 Zn2+ activates Morganella morganii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.2 40000
-
2 * 40000, SDS-PAGE Citrobacter freundii
4.3.1.2 44000
-
2 * 44000, SDS-PAGE Morganella morganii
4.3.1.2 70000
-
gel filtration Morganella morganii
4.3.1.2 79000
-
gel filtration Citrobacter freundii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.2 L-threo-3-methylaspartate Citrobacter freundii
-
?
-
?
4.3.1.2 L-threo-3-methylaspartate Clostridium tetanomorphum
-
?
-
?
4.3.1.2 L-threo-3-methylaspartate Morganella morganii
-
?
-
?
4.3.1.2 additional information Clostridium tetanomorphum enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes ?
-
?
4.3.1.2 additional information Clostridium tetanomorphum NCIMB 11547 enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.2 Citrobacter freundii
-
Citrobacter sp. strain YG-0504
-
4.3.1.2 Clostridium tetanomorphum
-
NCIMB 11547
-
4.3.1.2 Clostridium tetanomorphum NCIMB 11547
-
NCIMB 11547
-
4.3.1.2 Morganella morganii
-
-
-
4.3.1.2 Morganella morganii YG-0601
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.2
-
Citrobacter freundii
4.3.1.2
-
Morganella morganii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.2 additional information
-
-
Citrobacter freundii
4.3.1.2 additional information
-
-
Clostridium tetanomorphum
4.3.1.2 additional information
-
-
Morganella morganii
4.3.1.2 162
-
-
Citrobacter freundii
4.3.1.2 296
-
-
Morganella morganii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.2 (2S,3S)-3-ethylaspartic acid
-
Citrobacter freundii ?
-
?
4.3.1.2 (2S,3S)-3-ethylaspartic acid
-
Clostridium tetanomorphum ?
-
?
4.3.1.2 (2S,3S)-3-ethylaspartic acid
-
Morganella morganii ?
-
?
4.3.1.2 3-chloroaspartate
-
Clostridium tetanomorphum chlorofumarate + NH3
-
?
4.3.1.2 3-chloroaspartate (2R,3S)-3-chloroaspartic acid Citrobacter freundii chlorofumarate + NH3
-
?
4.3.1.2 3-chloroaspartate (2R,3S)-3-chloroaspartic acid Morganella morganii chlorofumarate + NH3
-
?
4.3.1.2 3-chloroaspartate
-
Clostridium tetanomorphum NCIMB 11547 chlorofumarate + NH3
-
?
4.3.1.2 3-chloroaspartate (2R,3S)-3-chloroaspartic acid Morganella morganii YG-0601 chlorofumarate + NH3
-
?
4.3.1.2 ? + NH4Cl
-
Citrobacter freundii ?
-
?
4.3.1.2 ? + NH4Cl
-
Morganella morganii ?
-
?
4.3.1.2 ethylfumarate + NH3
-
Citrobacter freundii ?
-
?
4.3.1.2 ethylfumarate + NH3
-
Clostridium tetanomorphum ?
-
?
4.3.1.2 ethylfumarate + NH3
-
Morganella morganii ?
-
?
4.3.1.2 ethylfumarate + NH3
-
Morganella morganii YG-0601 ?
-
?
4.3.1.2 L-threo-3-methylaspartate r Citrobacter freundii mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Clostridium tetanomorphum mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Morganella morganii mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter freundii mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Clostridium tetanomorphum mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Morganella morganii mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Clostridium tetanomorphum NCIMB 11547 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Clostridium tetanomorphum NCIMB 11547 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Morganella morganii YG-0601 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Morganella morganii YG-0601 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Citrobacter freundii ?
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Clostridium tetanomorphum ?
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Morganella morganii ?
-
?
4.3.1.2 additional information
-
Citrobacter freundii ?
-
?
4.3.1.2 additional information
-
Clostridium tetanomorphum ?
-
?
4.3.1.2 additional information
-
Morganella morganii ?
-
?
4.3.1.2 additional information enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes Clostridium tetanomorphum ?
-
?
4.3.1.2 additional information
-
Clostridium tetanomorphum NCIMB 11547 ?
-
?
4.3.1.2 additional information enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes Clostridium tetanomorphum NCIMB 11547 ?
-
?
4.3.1.2 additional information
-
Morganella morganii YG-0601 ?
-
?

Subunits

EC Number Subunits Comment Organism
4.3.1.2 dimer
-
Clostridium tetanomorphum
4.3.1.2 dimer 2 * 44000, SDS-PAGE Morganella morganii
4.3.1.2 dimer 2 * 40000, SDS-PAGE Citrobacter freundii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.2 45
-
maximal activity in the deamination reaction of L-threo-3-methylaspartate Morganella morganii
4.3.1.2 50
-
maximal activity in the deamination reaction of L-threo-3-methylaspartate Citrobacter freundii
4.3.1.2 55
-
-
Clostridium tetanomorphum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.1.2 additional information
-
-
Citrobacter freundii
4.3.1.2 additional information
-
-
Morganella morganii
4.3.1.2 45
-
pH 7.0, 10 min, 0.1 M potassium phosphate buffer, deamination reaction of (2S,3S)-3-methylaspartate, 5% loss of activity Citrobacter freundii
4.3.1.2 45
-
pH 7.0, 10 min, 0.1 M potassium phosphate buffer, deamination reaction of (2S,3S)-3-methylaspartate, 5% loss of activity Morganella morganii
4.3.1.2 50
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 50% loss of activity Citrobacter freundii
4.3.1.2 50
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 50% loss of activity Morganella morganii
4.3.1.2 55
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, most of activity is lost Citrobacter freundii
4.3.1.2 55
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, most of activity is lost Morganella morganii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.2 8
-
maximal activity in TRIS/HCl buffer in the amination reaction of mesaconate Morganella morganii
4.3.1.2 8.5
-
maximal activity in TRIS/HCl buffer in the amination reaction of mesaconate Citrobacter freundii
4.3.1.2 9.7
-
maximal activity in ethanolamine/HCl buffer in the deamination reaction of L-threo-3-methylaspartate Citrobacter freundii
4.3.1.2 9.7
-
maximal activity in ethanolamine/HCl buffer in the deamination reaction of L-threo-3-methylaspartate Morganella morganii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.1.2 6 11 pH 6.0: 10% of maximal activity, pH 11.0: 40% of maximal activity in the amination reaction of mesaconate Morganella morganii
4.3.1.2 7 11 pH 7.0: about 10% of maximal activity, pH 11.0: about 80% of maximal activity in the deamination reaction of L-threo-3-methylaspartate Morganella morganii
4.3.1.2 7 11 pH 7.0: about 25% of maximal activity, pH 11.0: about 5% of maximal activity, in the deamination reaction of L-threo-3-methylaspartate Citrobacter freundii
4.3.1.2 7 11 pH 7.0: about 10% of maximal activity, pH 11.0: about 90% of maximal activity in the amination reaction of mesaconate Citrobacter freundii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.3.1.2 additional information
-
-
Citrobacter freundii
4.3.1.2 additional information
-
-
Morganella morganii
4.3.1.2 5 10 stable between, pH 5.0: inactivated rapidly below Citrobacter freundii
4.3.1.2 6 8.5 stable between, pH 5.0: inactivated rapidly below Morganella morganii